EnzyChrom™ Acetaldehyde Assay Kit

Protocol SDS

Application

• For quantitative determination of acetaldehyde enzyme activity and evaluation of drug modulators.

Key Features

• Fast and sensitive. Linear detection range (20µL sample): 2µM to 2 mM acetaldehyde in a 96-well plate assay.
• High-throughput.The procedure involves adding a single working reagent and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed.
• Convenient.Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD565nm

Samples

• Biological samples (e.g. plasma, serum, urine, tissue, and culture media) and food/beverage samples (e.g. wine, coffee, and juice)

Species

• All

Procedure

• 30 min

Size

• 100 tests

Detection Limit

• 2 µM

Shelf Life

• 6 months

More Details

ACETALDEHYDE (CH₃CHO) is one of the most widely occurring aldehydes in nature and is commonly used in industry. Acetaldehyde, a metabolic byproduct of ethanol in the liver, is toxic to the human body and is rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. People with a deficiency of aldehyde dehydrogenase accumulate acetaldehyde when consuming alcohol and this accumulation results in facial and body flushing often referred to as “Asian flush syndrome.” Build up of acetaldehyde has also been associated with the effects of hangovers from alcohol consumption. Although classified as a carcinogen, acetaldehyde is naturally found in many foods and beverages such as ripe fruit, coffee, and wine. BioAssay Systems colorimetric acetaldehyde assay kit is based on aldehyde dehydrogenase catalyzed oxidation of acetaldehyde, in which the formed NADH reduces a formazan reagent. The intensity of the product color, measured at 565 nm, is directly proportional to the acetaldehyde concentration in the sample.

How specific is this assay for acetaldehyde?

The assay mechanism uses the enzyme aldehyde dehydrogenase to quantify acetaldehyde in the sample. The enzyme will also oxidize other aldehydes in the sample (i.e. propionaldehyde). Therefore, the assay will not distinguish between acetaldehyde and other aldehydes present in the sample. Consideration should be taken if high levels of other aldehydes are expected in the sample being tested.

How long are the diluted standards good for?

Acetaldehyde is a rather volatile chemical, so proper storage of the standard is vital. Only bring the 3 M Standard to room temperature for time needed to prepare standards. Return to -20°C within 30 minutes of thawing. Be sure to keep the Standard and standard dilutions on ice or refrigerated with the tube caps closed during assay procedure. The diluted standards for the colorimetric assay (4 mM, 2.4 mM, 1.2 mM, and 0 mM) and the fluorimetric assay (60 µM, 36 µM, 18 µM, and 0 µM) should be prepared fresh before each assay and used within 10 minutes of preparing.

What temperature do I run the assay at?

The assay may be run at room temperature (25°C) or 37°C.

No citations for this new product. Please check back later. You may click here to check if citations are available, but are not listed here yet.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.