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QuantiChrom™ Zinc Assay Kit
Application
- Determination of zinc in serum, plasma (no EDTA), urine, saliva, wastewater, soil, etc, and zinc metabolism.
Key Features
- Sensitive and accurate. Uses 50 μL samples. Detection range 0.05 μM (0.32 μg/dL) to 10 μM (65 μg/dL) zinc in 96-well assay format.
- Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
- Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay formats possible.
Method
- OD425nm
Samples
- Serum, plasma (non-EDTA), urine, saliva, wastewater, soil
Species
- All
Procedures
- 30 min
Size
- 250 tests in 96-well plate
Detection Limit
- 0.05 μM (0.32 μg/dL)
Shelf Life
- 6 months
More Details
Zinc is an essential trace element and plays many key roles in metabolism. It is required for the activity of more than 300 enzymes, the structure of many proteins, and control of genetic expression. Zinc status affects basic processes of cell division, growth, differentiation, development, performance and aging through its requirement for synthesis and repair of DNA, RNA and protein. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Zinc deficiency has now been recognized to be associated with many diseases such as malabsorption syndrome, chronic liver disease, chronic renal disease, sickle cell disease, diabetes, malignancy.Simple, direct and automation-ready procedures for measuring zinc concentration in biological samples are highly desirable in Research and Drug Discovery. BioAssay Systems’ zinc assay kit is designed to measure zinc directly in biological samples without any pretreatment. The present method utilizes a chromogen that forms a colored complex specifically with zinc. The intensity of the color, measured at 425 nm, is directly proportional to the zinc concentration in the sample.Our plate reader does not have a filter for 425 nm, can I use 405nm or 450 nm instead?
No, the zinc assay must be measured between 419 nm and 426 nm. Outside of this narrow range the assay will not work.
How many standards (to generate a graph) should I include in the assay?
Because this assay is not linear from 0.12 to 10 μM, a minimum of four points in the standard curve is recommended for each assay.
Do I need to dilute urine samples for this assay?
No, the zinc concentration in urine is sufficiently low.
What is the difference between this kit and your previous kit DIZN-250?
DIZN2-250 is a replacement for DIZN-250, which has been discontinued. This newer kit uses the same assay principle but combines the liquid Reagents B and C into one solid reagent for improved stability.
For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
$549.00
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.
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