QuantiChrom™ Magnesium Assay Kit

Protocol SDS

Application

• For quantitative determination of Mg²⁺ and evaluation of drug effects on magnesium metabolism.

Key Features

• Sensitive and accurate. Use as little as a 5 µL sample. Linear detection range 0.1 mg/dL (41 µM) to 3 mg/dL (1.2 mM) Mg²⁺ in a 96-well plate assay.
• Simple and high-throughput. The procedure involves the addition of two reagents and measuring OD500nm. Can be readily automated as a high-throughput assay for thousands of samples per day.
• Improved reagent stability and versatility. The optimized formulation has greatly enhanced the reagent and signal stability. Cuvet or 96-well plate assay.
• Low interference in biological samples. Assays can be directly performed in serum and urine samples.

Method

• OD500nm

Samples

• Biological, food, and beverage

Species

• All

Procedure

• 10 min

Size

• 250 tests

Detection Limit

• 0.1 mg/dL (41 µM)

Shelf Life

• 12 months

More Details

Magnesium (Mg) is one of the most abundant and essential minerals in mammals. Magnesium is involved in more than 300 biochemical reactions in the body and plays important roles in muscle and nerve functions, heart rhythm, immune system, and bone formation. Magnesium deficiency may lead to nausea, fatigue, muscle contractions, hypocalcemia, and hypokalemia. Simple, direct, and automation-ready procedures for measuring magnesium concentration in biological samples are becoming popular in Research and Drug Discovery. BioAssay Systems magnesium assay kit is designed to measure magnesium directly in biological samples without any pretreatment. A calmagite dye in the kit forms a colored complex specifically with magnesium. The intensity of the color, measured at 500 nm, is directly proportional to the magnesium concentration in the sample. The optimized formulation minimizes interference by potential substances.

What are typical optical density readings of deionized water and the magnesium standard in this assay?

The OD reading will vary with the experimental conditions and the OD reader used. In our laboratory, the OD(500 nm) for distilled water is typically around 0.300, and after the addition of EDTA basically unchanged, for instance 0.296. For the magnesium standard, the values are typically around 0.450 and 0.295, respectively.

What is the variability across replicated well samples?

The intra-assay variability is typically below 5%.

Do you recommend me to dilute my samples for the Magnesium Assay Kit?

Whether you need to dilute your sample depends on the nature of the sample and the expected concentration of Mg. Normal Mg levels in blood are 1.7-2.3 mg/dL and fall within the linear detection range of 0.1 to 3 mg/dL – dilution is thus not necessary. On the other hand for urine samples, normal values are typically higher, around 7.2 -10.8 mg/dL, and a dilution of the sample is necessary.

Zhao, J.-Y., et al (2020). Atherogenic diet accelerates ectopic mineralization in a mouse model of pseudoxanthoma elasticum. International Journal of Dermatology and Venereology, 3(2), 91-96. Assay: Magnesium in Mouse Serum.

Ando, I., et al (2020). Evaluation of stress status using the stress map for guide dog candidates in the training stage using variations in the serum cortisol with nerve growth factor and magnesium ions. Veterinary and Animal Science, 10, 100129. Assay: Magnesium in Dog Serum.

Park, J., et al (2021). Femtosecond laser-mediated anchoring of polymer layers on the surface of a biodegradable metal. Journal of Magnesium and Alloys. Assay: Magnesium in PLGA substrates.

Liu, M., et al (2019). Magnesium supplementation improves diabetic mitochondrial and cardiac diastolic function. JCI Insight, 4(1). Assay: Magnesium in mouse plasma.

Xu, T., Wang, X., Meng, L., Zhu, M., Wu, J., Xu, Y., Zhang, Y., & Zhang, W. (2020). Magnesium links starvation-mediated antibiotic persistence to atp. MSphere, 5(1). Assay: Magnesium in Staphylococcus aureus cells.

Villa-Bellosta, R. (2020). Dietary magnesium supplementation improves lifespan in a mouse model of progeria. EMBO Molecular Medicine, 12(10), e12423. Assay: Magnesium in Mouse plasma.

Liu, M., Jeong, E. M., Liu, H., Xie, A., So, E. Y., Shi, G. & Dudley Jr, S. C. (2019). Magnesium supplementation improves diabetic mitochondrial and cardiac diastolic function. JCI insight, 4(1). Assay: Magnesium in mice blood.

Ando, I., Karasawa, K., Yokota, S., Shioya, T., Matsuda, H., & Tanaka, A. (2017). Analysis of serum magnesium ions in dogs exposed to external stress: A pilot study. Open veterinary journal, 7(4), 367-374. Assay: Magnesium in dog serum.

Kingman, J., Uitto, J., & Li, Q. (2017). Elevated dietary magnesium during pregnancy and postnatal life prevents ectopic mineralization in Enpp1asj mice, a model for generalized arterial calcification of infancy. Oncotarget, 8(24), 38152. Assay: Magnesium in mice serum.

Ballester-Lozano GF et al. (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr. 114(5):713-26. Assay: magnesium in fish plasma.

Breiderhoff, T et al (2012). Deletion of claudin-10 (Cldn10) in the thick ascending limb impairs paracellular sodium permeability and leads to hypermagnesemia and nephrocalcinosis. Proceedings of the National Academy of Sciences 109(35): 14241-14246. Assay: Magnesium in mouse urine, serum.

Ullah, S., Khan, A. A., Khan, M. A., & Ahmed, I. (2012). Effects of gasoline engine emissions on health status of auto rickshaw drivers at DIR (L) Khyber Pukhtunkhwa. Toxicological & Environmental Chemistry 94(2): 411-422. Assay: Magnesium in human blood.

Elizondo MR, et al (2010). trpm7 regulation of in vivo cation homeostasis and kidney function involves stanniocalcin 1 and fgf23. Endocrinology 151(12):5700-9. Assay: Magnesium in zebrafish blood.

Pachikian BD, et al (2010). Changes in intestinal bifidobacteria levels are associated with the inflammatory response in magnesium-deficient mice. J Nutr. 140(3):509-14. Assay: Magnesium in mice serum.

LaRusso J, Li Q, Jiang Q, Uitto J (2009). Elevated dietary magnesium prevents connective tissue mineralization in a mouse model of pseudoxanthoma elasticum (Abcc6(-/-)). J Invest Dermatol 129:1388-1394. Assay: Magnesium in mouse serum, urine.

Pratihar, S., & Kundu, J. K. (2009). Increased Serum Magnesium and Calcium and their Regulation During Hibernation in the Indian Common Toad, Duttaphrynus melanostictus (Schneider, 1799). South American Journal of Herpetology 4(1): 51-54. Assay: Magnesium in toad serum.

Stippler, M et al (2007) Serum and Cerebrospinal Fluid Magnesium in Severe Traumatic Brain Injury Outcome. J Neurotrauma. 24(8): 1347-1354. Assay: Magnesium in human cerbrospinal fluid.

Kim TW et al (2006) Rapid production of milligram quantities of proteins in a batch cell-free protein synthesis system. J Biotechnol 124(2):373-80. Assay: Magnesium in cell free system protein.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.