QuantiFluo™ Protein Assay Kit

Protocol SDS

Application

• For quantitative determination of protein in various biological samples.

Key Features

• Simple and fast. A single reagent and assay completed within 5 min.
• Sensitive and wide detection range. Linear detection range of 1.3 – 1000 µg/mL BSA.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL360/450nm

Samples

• Biological, e.g. cells, tissue, proteins, peptide

Species

• All

Procedure

• 5 min

Size

• 200 tests

Detection Limit

• 1.3 µg/mL

Shelf Life

• 6 months

More Details

The protein or peptide is known as the “building blocks of life” and is one of the most important macromolecules in life science. Protein determination is a very common practice. Simple, direct and automation-ready procedures for measuring protein or peptide concentration are very desirable. BioAssay Systems QuantiFluo™ protein assay kit is based on an improved o-phthalaldehyde method. This reagent reacts with primary amines in protein or peptide and forms a blue fluorescent product, allowing detection of nanograms of proteins. The fluorescence intensity (λex/em = 360/450nm) is proportional to the protein concentration in the sample.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.