Protocol SDS

Application

• For quantitative determination of plasmin enzyme activity in biological samples.

Key Features

• Safe and sensitive. Non-radioactive assay. Use as little as 10 μL samples. Linear detection range in 96-well plate: 0.35 to 56.5 U/L activity.
• Fast and convenient. The procedure involves addition of a single working reagent and incubation for 30 min.
• High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.

Method

• OD405nm

Samples

• Serum and other biological samples

Species

• All

Procedures

• 30 min

Size

• 100 tests in 96-well plate

Detection Limit

• 0.35 U/L

Shelf Life

• 6 months

More Details

PLASMIN (3.4.21.7) is a serine protease that is a key enzyme in the process of fibrinolysis. Plasmin is released into circulation as its inactive form, plasminogen, which can be activated by enzymes such as urokinase. The plasmin/plasminogen system has been studied for its role in inflammation, degradation of the extracellular matrix, and wound healing. BioAssay Systems’ DPLM-100 kit provides a convenient colorimetric method to measure plasmin activity. In this assay, plasmin hydrolyzes a synthetic substrate to release p-nitroanilide (pNA), which absorbs at 405 nm. The increase in absorbance at 405 nm is directly proportional to the enzyme activity.