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EnzyChrom™ Phosphoglucomutase Assay Kit
Application
- For quantitative determination of phosphoglucomutase enzyme activity in biological samples.
Key Features
- Safe and sensitive. Non-radioactive assay. Linear detection range in 96-well plate: 0.5 to 20 U/L activity.
- Fast and convenient. The procedure involves addition of a single working reagent and incubation for 20 min. Room temperature assay. No 37°C incubator is needed.
- High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.
Method
- OD460nm
Samples
- Serum, plasma, cell lysate, and other biological samples
Species
- All
Procedures
- 20 min
Size
- 100 tests in 96-well plate
Detection Limit
- 0.5 U/L
Shelf Life
- 6 months
More Details
PHOSPHOGLUCOMUTASE (E.C. 5.4.2.2) is an enzyme which catalyzes the interconversion of glucose-1-phosphate (G1P) and glucose-6-phosate (G6P). Phosphoglucomutase (PGM) plays a crucial role in glycogen synthesis and degradation. PGM 1 deficiency can cause a rare congenital disorder of glycosylation. A decrease in PGM activity has also been linked with oxidative stress. In BioAssay Systems’ EPGM-100 assay, PGM converts G1P to G6P, which is then oxidized by glucose-6-phosphate dehydrogenase. The formed NADPH is coupled to a formazan chromogen. The increase in absorbance at 460 nm is directly proportional to phosphoglucomutase activity.Does this kit come with phosphoglucomutase?
No, this kit does not come with a phosphoglucomutase positive control.
What was the source of the enzyme used to develop this kit?
Phosphoglucomutase from rabbit skeletal muscle was used when developing this kit.
What cell count should I use when preparing cell lysate?
Our general guideline is to suspend about two million (2 x 10^6) harvested cells in 400 μL PBS on ice, then run a pilot test of the sample at different dilutions to determine the optimal one. When testing this kit with PANC-1 cell lysate, we used 650,000 cells/mL. However, the optimal number may vary depending on your cell line and samples.
For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
$539.00
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: Ice
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.
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