EnzyChrom™ Oxaloacetate Assay Kit

Protocol SDS

Application

• For quantitative determination of oxaloacetate and evaluation of drug effects on its metabolism.

Key Features

• Sensitive and accurate. Linear detection range: 7 to 400 µM oxaloacetate for colorimetric assays and 1 to 40 µM for fluorimetric assays.

Method

• OD570nm, or FL530/585nm

Samples

• Plasma, serum, tissue and culture media

Species

• All

Procedure

• 20 min

Size

• 100 tests

Detection Limit

• OD, FL: 4, 1 µM

Shelf Life

• 6 months

More Details

Oxaloacetate (OAA) is an intermediate in the citric acid cycle and participates in gluconeogenesis. OAA is formed by the oxidation of malate, by deamidation of aspartate or by condensation of CO₂ with pyruvate or phosphoenolpyruvate. BioAssay Systems’ Oxaloacetate Assay Kit provides a simple, direct, and automation-ready procedure for measuring oxaloacetate concentration. OAA is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the oxaloacetate concentration in the sample.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.