QuantiQuik™ Oxalate Quick Test Strips

Protocol SDS

Application

• For rapid, direct determination of oxalate concentrations in biological samples. For Research Use Only (not for medical decisions).Note: For urine samples, activated carbon (Catalog: CARB-10), cutoff filters or centrifuge (Catalog: CTFG) and 0.5M EDTA (Catalog: EDTA-150) are needed.

Key Features

• Fast and sensitive. Use of 10 µL sample. Semi-quantitative measurement between 0-1.5 mg/dL or 0-170 µM (undiluted) Oxalate.
• Convenient. No expensive plate or cuvette readers required.
• Sample treatment and assay can be performed in under 10 minutes.

Method

• Visual

Samples

• Serum, plasma, urine, fruits or vegetables, etc

Species

• All

Procedure

• Approximately 10 min

Size

• 10 tests

Shelf Life

• 6 months

More Details

Oxalate, or Oxalic Acid, is a metabolic breakdown product of the Kreb’s Cycle in eukaryotes and the glyoxylate cycle in other microorganisms. Typically present in the urine of humans and other mammals, oxalate concentration can be used as a measure of kidney function. A high level of oxalate is an indicator for kidney stones, which are primarily made of the insoluble salt calcium oxalate. Measuring oxalate is more accurate than measuring calcium as a marker for kidney stones because calcium is excreted at high concentrations even in normal urine.BioAssay Systems’ QuantiQuik™ Oxalate Quick Test Strips are based on the enzymatic oxidation of oxalate coupled with a color reaction in one step. The intensity of product color is directly proportional to the oxalate concentration in the sample.

What samples have you tested?

The strips have been tested on serum, plasma, and urine. We have also tested saliva and would not recommend testing it with this product.

My sample turns the strip very dark blue, how can I determine the oxalate concentration?

Very dark blue indicates that the diluted sample concentration is greater than 1.5 mg/dL Oxalate. To obtain a more accurate concentration, the sample should be further diluted and retested. For example, if the sample was tested undiluted, try diluting 5× or 10×.

Can I store unused reagents for future use?

Yes, unused reagents can be stored according to the assay protocol. The strips should be stored in the refrigerator (4°C).

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.