Application

• For rapid, direct determination of L-Glutamate (monosodium glutamate or MSG) concentrations in food and beverage samples as well as biological samples.
  Note: Requires access to pipettes for dilution. We offer pipettes that can be purchased separately: 20 uL Pipettes (Catalog: TP-20) or 100 uL Pipettes. (Catalog: TP-100)

Key Features

• Fast and sensitive. Use of 20 or 100 µL sample. Semi-quantitative measurement between 0-300 mg/L (undiluted) L-Glutamate.
• Convenient. No extra equipment required.
• Sample treatment and assay can be performed in under 15 minutes.

Method

• Visual

Samples

• Soups, sauces, milk, etc

Species

• All

Procedure

• Approximately 15 min

Size

• 10 tests

Shelf Life

• 6 months

More Details

• GLUTAMATE, is an important chemical in general metabolism. It is also widely used as a flavor enhancer in the food industry. Consumption of foods containing monosodium glutamate (MSG) have been known to cause headaches, flushing, sweating, nausea, and more. Glutamate is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, autism, and Alzheimer’s disease. BioAssay Systems QuantiQuik™ L-Glutamate Quick Test Strips are based on Glutamate dehydrogenase catalyzed oxidation of glutamate in which the formed NADH reduces a chromogenic reagent. The intensity of the product color is directly proportional to the glutamate concentration in the sample.

What samples have you tested?

The strips have been tested on instant soup broth, restaurant Pho broth, soy sauce, fish sauce, 2% milk, plasma, serum, and urine.

What is the recommended dilution for samples with low levels of Glutamate?

For samples with low levels of Glutamate (e.g. plasma, urine, serum, etc) we recommend diluting samples 2×. To do this, carefully transfer 400 µL of sample to the Sample Development Tube. If a pipetteman is not available, we have 400 µL single use transfer pipettes that can be purchased separately (Cat No. TP400). Please note that samples need to have at least 0.1 – 0.2 mM Glutamate to be detected by the strip.

My sample turns the strip very dark purple, how can I determine the L-glutamate concentration?

Very dark purple indicates that the diluted sample concentration is greater than 2 mM. To obtain a more accurate concentration, the sample should be further diluted and retested. For example, if the sample was diluted 5×, try diluting 21×.

I don’t have access to a pipetteman. How can I accurately measure out my samples?

We offer exact volume transfer pipettes as an accessory. For samples requiring a 21× dilution, order the 20 µL transfer pipettes (TP20). For samples requiring a 5× dilution, order the 100 µL transfer pipettes (TP100). For samples requiring a 210× dilution, order the 20 µL and 100 µL transfer pipettes.

The color seen on my strip appears to be a different shade than the color chart on the strip bag. What could be the problem?

Fluorescent lights can have a green cast so it is best to view strips under incandescent or natural lighting for optimal color matching.

Can I store unused reagents for future use?

Yes, unused reagents can be stored according to the assay protocol. The strips should be kept in a dry, cool location and protected from light.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.