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EnzyChrom™ NADP+/NADPH Assay Kit
Application
- For quantitative determination of NADP+/NADPH concentrations and ratios in cell or tissue extracts.
Key Features
- Sensitive and accurate. Detection limit 0.1 µM, linearity up to 10 µM NADP+/NADPH in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Method
- Colorimetric Determination at 565 nm
Samples
- Cells, Tissue
Species
- human, mouse, rat and more
Procedure
- 30 min
Size
- 100 tests
Detection Limit
- 0.1 µM
Shelf Life
- 6 months
More Details
- Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.
Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable. BioAssay Systems’ EnzyChromTM NADP+ /NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and is not interfered by NAD+/NADH. Our assay is a convenient method to measure NADP, NADPH and their ratio.
When tissue is used, should it be freshly obtained? Or is -80°C storage ok?
If direct sample processing is not possible, we recommend snap freezing tissue samples in liquid nitrogen and keeping them either in liquid nitrogen or at -80°C until further processing.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
$479.00
For orders of 10 or more kits, please call +1-510-782-9988×1 or email us for best pricing and/or bulk order.
Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt
Why BioAssay Systems?
Technical support provided by the very scientists that develop the assays.
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.
Because we develop and manufacture the products, our prices are lower than competitors on the market
With over 200 different products, acquire all your assay kit needs in one order.
Products used by clients worldwide with distributors in over 60 countries.
