EnzyChrom™ Monoamine Oxidase Inhibitor Screening Kit

Protocol SDS

Application

• For evaluation and high-throughput screen (HTS) of monoamine oxidase modulators.

Key Features

• Homogeneous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
• Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL530/585nm

Samples

• Compounds that affect monoamine oxidase (MAO) activity

Species

• All

Procedure

• 35 min

Size

• 100 tests

Shelf Life

• 6 months

More Details

MONOAMINE OXIDASES (MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. Two isoforms of MAO exist, MAO-A and MAO-B, with different inhibitor selectivity and tissue distribution. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAOs in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drugs prescribed for the treatment of depression, Parkinson’s, and Alzheimer’s diseases. BioAssay Systems MAO Inhibitor Screening Assay Kit provides a convenient fluorimetric means to screen for MAO enzyme inhibitors. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H₂O₂, which is determined by a fluorimetric method (λ_em/ex = 585/530 nm). The assay is simple, sensitive, stable, and high-throughput adaptable.

Does the kit come with MAO-A and MAO-B enzymes or control MAO inhibitors?

The kit does not comes with either MAO enzyme. However, it comes with a clorgyline and pargylineas control inhibitors for MAO A and MAO B respectively.

How do I store the kit?

Store kit at -20°C for long term storage.

What species of MAO was the kit optimized for?

The kit was optimized for human MAO.

What if I am interested in testing inhibition of a species besides human?

We recommend that you experimentally determine the K_m and then adjust the volume of substrate in the Working Reagent so that the final concentration of the substrate in the 100µL Reaction Volume is near the K_m.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.