EnzyChrom™ Maltose Assay Kit

Protocol SDS

Application

• For quantitative determination of maltose and evaluation of drug effects on its metabolism.

Key Features

• Fast and sensitive. Use of 10 µL sample. Linear detection range from 2 to 500 µM maltose for colorimetric assays and 1 to 50 µM for fluorimetric assays.
• Convenient. The procedure involves adding a single working reagent and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed.
• High-throughput. “Add-mix-read” type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

• OD570nm, or FL530/585nm

Samples

• Serum, urine, food, and beverages, etc

Species

• All

Procedure

• 60 min

Size

• 100 tests

Detection Limit

• 2 µM

Shelf Life

• 6 months

More Details

MALTOSE(C₁₂H₂₂O₁₁) is a disaccharide, composed of two glucose units linked by an α bond. It is produced from the hydrolysis of glycogen or starch, serving as a source of energy for plants and animals. Maltose can be found in foods such as grains, and other processed products. BioAssay Systems maltose assay provides a simple, one-step assay for measuring maltose. In this assay, maltose is converted to two glucose, which are then oxidized to form a colored product. The color intensity of the product at 570 nm or fluorescence at λex/em = 530/585 nm is directly proportional to maltose concentration in the sample.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.