Yes, you can increase the enzyme incubation step from 30 minutes to a time that provides detectable activity. The incubation time with the detection reagent will remain the same (20 minutes). Be sure to adjust the incubation time included in the calculation to represent the time you actually used.
Yes, you can use this kit to determine the enzymatic parameters of your Glutaminase. The kit comes with a 20 mM Stock solution of the Glutamine substrate. You can use different dilutions of the substrate to generate the enzymatic parameters of your Glutaminase.
Our kit does not include enzyme. The customer needs to provide their enzyme of choice for this assay.
For more detailed product information and questions, please feel free to email us or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.