QuantiChrom™ γ-Glutamyl Transferase Assay Kit

Protocol SDS

Application

• For quantitative determination of γ-glutamyl transferase enzyme activity in biological samples.

Key Features

• Safe and sensitive. Non-radioactive assay. Use as little as 10 μL of sample. Linear detection range in 96-well plate: 0.7 to 200 U/L activity.
• Fast and convenient. The procedure involves addition of a single ready-to-use reagent and incubation for 30 min. Room temperature assay. No 37°C incubator is needed.
• High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.

Method

• OD405nm

Samples

• Serum, plasma, cell lysate, and other biological samples

Species

• All

Procedures

• 30 min

Size

• 100 tests in 96-well plate

Detection Limit

• 0.7 U/L

Shelf Life

• 6 months

More Details

γ-GLUTAMYL TRANSFERASE (GGT, E.C. 2.3.2.2) is an enzyme that catalyzes the transfer of a glutamyl group to an acceptor, such as a peptide or amino acid. GGT plays a major role in the breakdown of glutathione and is found in many tissues, but is more commonly found in the liver. Elevated levels of GGT may indicate liver disease or damage to the bile ducts, and in clinical practice, it is often tested along with other liver function tests. BioAssay Systems’ DGGT-100 kit provides a convenient colorimetric method to measure GGT activity. In this assay, GGT hydrolyzes a synthetic substrate to release p-nitroanilide (pNA), which absorbs at 405 nm. The increase in absorbance at 405 nm is directly proportional to the enzyme activity.

Does this kit come with a positive control?

No, this kit does not include GGT enzyme. This needs to be supplied by the user.

I am encountering high assay background, is this an issue?

There may be increase in background signal of the Reagent over time. However, the calculated results should still be fine since the background is subtracted out when calculating the ΔOD (30 – 0 min).

The results for my samples are not within the reference ranges for GGT.

Please note that the unit definition used for this assay is based on the μmol of pNA generated, which can be different from the units used by clinical reference ranges that are often listed in IU (international units). Reference ranges may also vary depending on the laboratory. Please also keep in mind that this kit is for research use only, and not to be used for diagnostic purposes.

For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.