The concentration of the NADH solution is 10 mM. The concentration of NADH in the final reaction volume (V = 220 μL) is calculated by accounting for the dilution steps when preparing the working reagent, i.e. 4 μL/206μL in the first step and 200 mL/220 mL in the second step. This is the final initial concentration of NADH in the reaction.
To calculate the activity of the sample, i.e. µmoles NADH consumed / (L x min), one has to divide by 5 min incubation time and multiply by the dilution factor of the sample which is 11.
Multiplying all these constant values together equals 388.
There is no need for a path length correction, because the sample and the standard wells contain the same volume.
Andres-Hernando, A., et al (2020). Deletion of fructokinase in the liver or in the intestine reveals differential effects on sugar-induced metabolic dysfunction. Cell Metabolism, 32(1), 117-127.e3 Assay: ASP in mouse serum.
Fang, W., et al (2020). Cathepsin B deficiency ameliorates liver lipid deposition, inflammatory cell infiltration, and fibrosis after diet-induced nonalcoholic steatohepatitis. Translational Research: The Journal of Laboratory and Clinical Medicine, 222, 28-40. Assay: ASP in mouse plasma.
Andres-Hernando, A., et al (2021). Vasopressin mediates fructose-induced metabolic syndrome by activating the V1b receptor. JCI Insight, 6(1). Assay: ASP in mouse plasma.
Seo, B., et al (2020). Roseburia spp. Abundance associates with alcohol consumption in humans and its administration ameliorates alcoholic fatty liver in mice. Cell Host & Microbe, 27(1), 25-40.e6. Assay: ASP in mouse serum.
Ishak Gabra, M. B., et al (2020). Dietary glutamine supplementation suppresses epigenetically-activated oncogenic pathways to inhibit melanoma tumour growth. Nature Communications, 11(1), 3326. Assay: ASP in mouse serum.
Xu, W., et al (2020). Lyp-1-modified oncolytic adenoviruses targeting transforming growth factor beta inhibit tumor growth and metastases and augment immune checkpoint inhibitor therapy in breast cancer mouse models. Human Gene Therapy, 31(15-16), 863-880. Assay: ASP in mouse serum.
Shin, M. K., Yang, S.-M., & Han, I.-S. (2020). Capsaicin suppresses liver fat accumulation in high-fat diet-induced NAFLD mice. Animal Cells and Systems, 24(4), 214-219. Assay: ASP in mouse plasma.
Andres-Hernando, A., et al (2020). Sugar causes obesity and metabolic syndrome in mice independently of sweet taste. American Journal of Physiology. Endocrinology and Metabolism, 319(2), E276-E290. Assay: ASP in mouse serum.
Sae-Khow, K., et al (2020). Pathogen-associated molecules from gut translocation enhance severity of cecal ligation and puncture sepsis in iron-overload beta-thalassemia mice. Journal of Inflammation Research, 13, 719-735. Assay: ASP in mouse serum.
Amor, C.,et al (2020). Senolytic CAR T cells reverse senescence-associated pathologies. Nature, 583(7814), 127-132. Assay: ASP in mouse serum.
Choi, E.-Y., et al (2020). Water extract of artemisia annua l. Exhibits hepatoprotective effects through improvement of lipid accumulation and oxidative stress-induced cytotoxicity. Journal of Medicinal Food, 23(12), 1312-1322 Assay: ASP in human hepatocarcinoma cells.
Simion, V., et al (2020). A macrophage-specific lncRNA regulates apoptosis and atherosclerosis by tethering HuR in the nucleus. Nature Communications, 11(1), 6135. Assay: ASP in mouse serum.
Andreata, F., et al (2020). Co-administration of H-ferritin-doxorubicin and Trastuzumab in neoadjuvant setting improves efficacy and prevents cardiotoxicity in HER2 + murine breast cancer model. Scientific Reports, 10(1), 11425. Assay: ASP in mouse tissue.
Li, Yajuan, et al (2019). PTEN-induced partial epithelial-mesenchymal transition drives diabetic kidney disease. The Journal of clinical investigation 129.3. Assay: Aspartate transaminase in mice serum.
Hay, Charles E., et al (2018). Development and testing of AAV-delivered single-chain variable fragments for the treatment of methamphetamine abuse. PloS one 13.6: e0200060. Assay: Aspartate transaminase in mice serum.
Kamerkar, Sushrut, et al (2017). Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer. Nature 546.7659: 498. Assay: Aspartate transaminase in mice plasma.
Kim, Soo Hyun, et al (2017). Ezetimibe ameliorates steatohepatitis via AMP activated protein kinase-TFEB-mediated activation of autophagy and NLRP3 inflammasome inhibition. Autophagy 13.10: 1767-1781. Assay: Aspartate transaminase in mice serum.
Sharabi, K et al (2017). Selective chemical inhibition of PGC-1alpha gluconeogenic activity ameliorates type 2 diabetes. Cell 169.1: 148-160. Assay: Aspartate transaminase in mice serum.
Wardah, J. Rahmahani, and T. Sopandi (2017). Effect of Phyllanthus buxifolius Leaf as a Feed Supplement on Liver Function and Haematological Response of Quail (Coturnix coturnix japonica) Challenged with Infectious Newcastle Disease Virus. International Journal of Poultry Science 16: 354-363. Assay: Aspartate transaminase in quail serum.
Weis, Sebastian, et al (2017). Metabolic adaptation establishes disease tolerance to sepsis. Cell 169.7: 1263-1275. Assay: Aspartate transaminase in mice plasma.
Seemann, Semjon, and Amelie Lupp (2016). Administration of AMD3100 in endotoxemia is associated with pro-inflammatory, pro-oxidative, and pro-apoptotic effects in vivo. Journal of biomedical science 23.1: 68. Assay: Aspartate transaminase in mouse serum.
Ballester-Lozano GF et al. (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr. 114(5):713-26. Assay: aspartate aminotransferase enzyme activity in fish.
Laura, BP et al. (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markers in gilthead sea bream (Sparus aurata). Aquaculture 454: 8-18. Assay: Aspartate transaminase in fish plasma.
Seemann, S and A Lupp (2015). Administration of a CXCL12 Analog in Endotoxemia Is Associated with Anti-Inflammatory, Anti-Oxidative and Cytoprotective Effects In Vivo. PLoS One 10(9): e0138389. Assay: Aspartate transaminase in mouse serum.
Jiang, S., et al (2014). Effect of perches on liver health of hens. Poultry science, 93(7), 1618-1622. Assay: Aspartate transaminase in hen serum.
Vives, V et al (2014). Pharmacological inhibition of Dock5 prevents osteolysis by affecting osteoclast podosome organization while preserving bone formation. Nature Communications 6: 6128. Assay: Aspartate transaminase in mouse serum.
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