EnzyChrom™ Galactose Assay Kit

Protocol SDS

Application

• For quantitative determination of galactose and evaluation of drug effects on its metabolism.

Key Features

• Use as little as 20 µL samples. Linear detection range in 96-well plate: 10 to 1000 µM galactose for colorimetric assays and 10 to 100 µM for fluorimetric assays.

Method

• OD570nm, or FL530/585nm

Samples

• Serum, plasma, urine, saliva, milk, culture medium, food, beverage, agriculture, etc

Species

• All

Procedure

• 20 min

Size

• 100 tests

Detection Limit

• 10 µM

Shelf Life

• 12 months

More Details

Galactose (C₆H₁₂O₆) is a monosaccharide that is found in dairy products, sugar beets, gums, and mucilages. It is also synthesized in mammals, where it forms part of glycolipids and glycoproteins in several tissues. It forms the disaccharide lactose when combined with glucose. Simple, direct, and high-throughput assays for galactose determination find wide applications. BioAssay Systems assay uses specific enzyme-coupled reactions to form a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the galactose concentration in the sample.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Xue, H. et al (2020). Lactose-Induced Chronic Diarrhea Results From Abnormal Luminal Microbial Fermentation and Disorder of Ion Transport in the Colon. Frontiers in Physiology, 11. Assay: galactose in rat colon content.

Shi, Jia, Yu Fu, and Xin-Huai Zhao (2018). Effects of Maillard-type caseinate glycation on the preventive action of caseinate digests in acrylamide-induced intestinal barrier dysfunction in IEC-6 cells. RSC advances 8.66: 38036-38046. Assay: Galactose in caseinate.

Sun, Ya-ting, et al. (2013). Development of a fed-batch cultivation for antibody-producing cells based on combined feeding strategy of glucose and galactose. Biochemical Engineering Journal 81: 126-135. Assay: Galactose in human culture medium.

Ya-ting, S et al (2013). Development of a fed-batch cultivation for antibody-producing cells based on combined feeding strategy of glucose and galactose. Biochemical Engineering Journal 81: 126-135. Assay: Galactose in cells.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.