QuantiQuik™ Fecal Occult Blood Test Strips

Protocol SDS

Application

• For fecal occult blood determination in human, mouse, rabbit, chicken, goat and pig feces samples.

Key Features

• Sensitive. Allows detection of very low occult blood (e.g. 0.15mg hemoglobin/g in mouse) in feces samples.
• Convenient. No sophisticated laboratory equipment is required.
• Sample treatment and assay can be performed in under 15 minutes.

Method

• Visual

Samples

• Feces samples

Species

• Human, mouse, rabbit, chicken, goat and pig

Procedures

• 10 min

Size

• 10 tests

Detection Limit

• 0.15mg hemoglobin/g in mouse

Shelf Life

• 6 months

More Details

FECAL OCCULT BLOOD (FOB) represents hidden blood in feces. The presence of FOB indicates GI tract hemorrhage. GI tract hemorrhage is a common symptom of colorectal cancer and GI tract inflammation, among other health complications. Therefore, determining the presence of FOB in mammal feces sample is critical for health care. This product can be applied to mammals including humans, goats, dogs, rodents and birds. BioAssay Systems’ Fecal Occult Blood Test (FOBT) provides a quick, simple, convenient and ultrasensitive method for detection of blood in feces samples through a red-ox indicator. As hemoglobin in the feces sample oxidizes the substrate, the red-ox indicator changes from light-brown to blue. The development of a blue color indicates the presence of blood in the sample. The intensity of the blue color correlates to the amount of occult blood in the sample.

Can I quantify the amount Hemoglobin in the feces sample?

No, this test was designed to indicate if the feces sample contains blood or not.

My test strip turned to green color after a long term storage. Can this test strip be used for an experiment?

Yes, the test strip still can be applied to a Fecal Occult Blood Test. The presence of green color on the strip is caused by partial decomposition of redox indicator on the strip. However, we have tested that the test strip turned to green also can detect the presence of the blood at the detection limit.

I don’t have access to a homogenizer. How can I effectively homogenize the feces samples?

The feces sample can be homogenized with a tooth pick by vigorously shaking for 1 minute in the deionized water.

For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.