EnzyChrom™ Coenzyme A Assay Kit

Protocol SDS

Application

• For quantitative determination of coenzyme A (CoA) and evaluation of drug effects on CoA metabolism.

Key Features

• Sensitive. Use 10 µL samples. Linear detection range: colorimetric assay 5 – 1000 µM, fluorimetric assay 3 – 100 µM CoA.
• Convenient. Room temperature “mix-and-read” procedure can be readily automated for high-throughput assay of thousands of samples per day.

Method

• OD570nm, or FL530/585nm

Samples

• Biological

Species

• All

Procedure

• 60 min

Size

• 100 tests

Detection Limit

• 3 µM

Shelf Life

• 6 months

More Details

Coenzyme A (CoA) is involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many others. One of CoA’s most crucial roles is the carrying and transferring of acyl groups. BioAssay Systems method provides a simple, two-step and high-throughput assay for measuring CoA. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H₂O₂. The resulting H₂O₂ reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the CoA concentration in the sample.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Takahashi, Takayuki, Yukitoshi Mine, and Tadashi Okamoto (2018). 2, 3-Dimethoxy-5-methyl-p-benzoquinone (Coenzyme Q0) Disrupts Carbohydrate Metabolism of HeLa Cells by Adduct Formation with Intracellular Free Sulfhydryl-Groups, and Induces ATP Depletion and Necrosis. Biological and Pharmaceutical Bulletin 41.12: 1809-1817. Assay: Coenzyme A in human cells.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.