QuantiChrom™ Cobalt Assay Kit

Protocol SDS

Application

• For Direct determination of cobalt ions in aqueous samples.

Key Features

• Sensitive and accurate. Linear detection range from 1.4 µM to 200 µM cobalt ions in a 96-well plate assay.
• Simple and high-throughput. The procedure involves the addition of a single working reagent with immediate color development. Can be readily automated as a high-throughput assay for thousands of samples per day.
• Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent stability. Cuvette or 96-well plate assay.

Method

• OD485nm

Samples

• Aqueous samples

Species

• All

Procedures

• 10 min

Size

• 100 tests in a 96-well plate

Detection Limit

• 1.4µM

Shelf Life

• 6 months

More Details

Cobalt is an essential micronutrient for all multicellular organisms as the active center of cobalamin, such as vitamin B-12, which is essential for plants and animals. Cobalt is also a micronutrient for bacteria, algae, and fungi. Despite being an important micronutrient, excess cobalt in the environment can be deleterious to life leading to cardiomyopathy in humans and necrosis in plants.Simple, direct, and automation-ready procedures for measuring cobalt concentrations find wide applications in research and environmental monitoring. BioAssay Systems’ cobalt assay kit is designed to measure total cobalt directly in aqueous samples without any pretreatment. The intensity of the color, measured at 485nm, is directly proportional to the cobalt concentration in the sample.

Do other ions interfere with the detection of Cobalt ions?

The metal ions Fe²⁺, Cu²⁺, Zn²⁺, Mn²⁺, Mg²⁺, Al³⁺, Mo²⁺, Pb²⁺ and W²⁺ exhibit less than 10% interference in this assay. Ni²⁺ concentrations >10% of Co²⁺ concentration should be avoided. Excessive Ca²⁺ may cause precipitation to occur.

Will increasing the incubation time with the Detection Reagent increase the sensitivity of the assay?

No, increasing the incubation time beyond about 15-20 minutes will only result in the formation of a precipitate. The color formation with Cobalt ion is almost instantaneous, so a longer incubation time is not required.

For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.