EnzyFluo™ Bile Acid Assay Kit

Protocol SDS

Application

• For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism.

Key Features

• Safe. Non-radioactive assay.
• Sensitive and accurate. Linear detection range of 1 – 150 µM bile acids.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL530/585nm

Samples

• Serum, plasma, urine, and other biological samples

Species

• All

Procedure

• 20 min

Size

• 100 tests

Detection Limit

• 1 µM

Shelf Life

• 6 months

More Details

BILE ACIDS. Twelve different types of bile acids are typically found in mammals, among them two primary types are cholic acid and chenodeoxycholic acid. These can be dehydroxylated into secondary bile acids. Finally, these four can be conjugated to either taurine or glycine creating 8 different conjugated bile acids. Bile acid levels in feces, blood, urine, and bile can be used as markers for various diseases such as hyperlipidemia, cholestasis, gallstones, colon cancer, etc. Bile acids also exist in a sulfate salt form known as bile acid-sulfates. Sulfation of bile acids increases their solubility and decreases intestinal absorption, thereby enhancing fecal and urinary excretion. This assay does not measure bile acid-sulfates, and measures only the twelve non-sulfated bile acids. BioAssay Systems’ Bile Acid Assay Kit provides a convenient fluorimetric means to measure total bile acids in biological samples. In the assay, 3α-hydroxysteroid dehydrogenase reacts with all twelve bile acids, converting NAD to NADH, which reduces a probe to a highly fluorescent product. The resulting fluorescence intensity (λex/em = 530/585 nm) is linear to the bile acid concentration in the sample.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Ommati, M. M., et al (2020) Oral administration of thiol-reducing agents mitigates gut barrier disintegrity and bacterial lipopolysaccharide translocation in a rat model of biliary obstruction. Current Research in Pharmacology and Drug Discovery, 1, 10-18. Assay: Bile Acid in rats serum.

Ommati, M. M., Farshad, O., Niknahad, H., Arabnezhad, M. R., Azarpira, N., Mohammadi, H. R., Haghnegahdar, M., Mousavi, K., Akrami, S., Jamshidzadeh, A., & Heidari, R. (2019). Cholestasis-associated reproductive toxicity in male and female rats: The fundamental role of mitochondrial impairment and oxidative stress. Toxicology Letters, 316, 60-72. Assay: Bile Acid in rats serum, ovary and testes tissue.

Sano, M., et al (2020). Increase in the lipopolysaccharide activity and accumulation of gram-negative bacteria in the stomach with low acidity. Clinical and Translational Gastroenterology, 11(7). Assay: Bile Acid in human gastric fluid.

Ommati, M. M.,et al (2020). N-acetyl cysteine treatment mitigates biomarkers of oxidative stress in different tissues of bile duct ligated rats. Stress (Amsterdam, Netherlands), 1-16. Assay: Bile Acid in rats serum and tissue.

Igarashi, Muneki, et al (2017). Alteration in the gastric microbiota and its restoration by probiotics in patients with functional dyspepsia. BMJ open gastroenterology 4.1: e000144. Assay: Bile Acid in human gastric fluid.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.