QuantiChrom™ Alcohol Dehydrogenase Assay Kit

Protocol SDS

Application

• For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism.

Key Features

• Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD565nm

Samples

• Biological (e.g. plasma, serum, urine, tissue, and culture media)

Species

• All

Procedure

• 30 min

Size

• 100 tests

Detection Limit

• 0.4 U/L

Shelf Life

• 6 months

More Details

ALCOHOL DEHYDROGENASE (ADH) is an oxidoreductase that catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the breakdown of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation. BioAssay System’s non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in an NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

No frequently asked questions for this new product. Please check back later.

For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Capri, K. M., et al. (2020). Effects of time of day and constant light on the behavioral responses and ethanol metabolism to acute alcohol administration in male Black Swiss mice. Biological Rhythm Research 51(4): 566-585. Assay: Alcohol Dehydrogenase in mice.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.