EnzyChrom™ Alanine Transaminase Assay Kit

Protocol SDS

Application

• For quantitative determination of alanine transaminase enzyme activity.

Key Features

• Sensitive. Linear detection range: 2-100 U/L.
• Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min.

Method

• OD340nm

Samples

• Serum, plasma etc

Species

• All

Procedure

• 10 min

Size

• 100 tests

Detection Limit

• 2 U/L

Shelf Life

• 6 months

More Details

Alanine Transaminase (ALT), also known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (SGPT), facilitates the conversion of alanine and a-ketoglutarate to pyruvate and glutamate. ALT plays an important role in gluconeogenesis and amino acid metabolism. ALT is found mainly in the liver, and, to a lesser extent, in kidney, heart, muscle, and pancreas tissues. Normal serum levels of ALT are low, and increased serum ALT activity is widely used as a marker for liver damage. Simple, direct and automation-ready procedures for measuring ALT activity find wide applications in research and drug discovery. BioAssay Systems ALT activity assay is based on the quantification of pyruvate produced by ALT. In this assay, pyruvate and NADH are converted to lactate and NAD by the enzyme lactate dehydrogenase (LDH). The decrease in NADH absorbance at 340 nm is proportional to ALT activity.

In regards to the ALT Assay Kit (EALT-100), do you guys sell a positive control?

We do not sell a positive control for this assay. Purified ALT is available from companies such as Sigma. Alternatively, serum samples could be used, for instance FBS (not heat inactivated).

Andres-Hernando, et al (2020). Deletion of Fructokinase in the Liver or in the Intestine Reveals Differential Effects on Sugar-Induced Metabolic Dysfunction. Cell metabolism, 32(1), 117-127. Assay: alanine transaminase in mouse plasma.

Fang, W. et al (2020). Cathepsin B Deficiency Ameliorates Liver Lipid Deposition, Inflammatory Cell Infiltration, and Fibrosis After Diet-Induced Nonalcoholic Steatohepatitis. Translational research: the journal of laboratory and clinical medicine, 222, 28-40. Assay: alanine transaminase in mouse plasma.

Visitchanakun, P. et al (2020). Gut Leakage Enhances Sepsis Susceptibility in Iron-Overloaded β-thalassemia Mice Through Macrophage Hyperinflammatory Responses. American journal of physiology. Gastrointestinal and liver physiology, 318(5), G966-G979. Assay: alanine transaminase in mouse serum.

Gabra, M. B. I. et al (2020). Dietary glutamine supplementation suppresses epigenetically-activated oncogenic pathways to inhibit melanoma tumour growth. Nature Communications, 11. Assay: alanine transaminase in mouse serum.

Jiang, S. et al (2020). Dojuksan ameliorates tubulointerstitial fibrosis through irisin-mediated muscle-kidney crosstalk. Phytomedicine: international journal of phytotherapy and phytopharmacology, 80, 153393. Assay: alanine transaminase in mouse plasma.

Xu, W. et al (2020). LyP-1-Modified Oncolytic Adenoviruses Targeting Transforming Growth Factor β Inhibit Tumor Growth and Metastases and Augment Immune Checkpoint Inhibitor Therapy in Breast Cancer Mouse Models. Human gene therapy, 31(15-16), 863-880. Assay: alanine transaminase in mouse serum.

Shin, M. K., Yang, S. M., & Han, I. S. (2020). Capsaicin suppresses liver fat accumulation in high-fat diet-induced NAFLD mice. Animal cells and systems, 24(4), 214-219. Assay: alanine transaminase in mouse plasma.

Andres-Hernando, A. et al (2020). Sugar causes obesity and metabolic syndrome in mice independently of sweet taste. American journal of physiology. Endocrinology and metabolism, 319(2), E276-E290. Assay: alanine transaminase in mouse serum.

Sae-Khow, K. et al (2020). Pathogen-Associated Molecules from Gut Translocation Enhance Severity of Cecal Ligation and Puncture Sepsis in Iron-Overload β-Thalassemia Mice. Journal of inflammation research, 13, 719-735. Assay: alanine transaminase in mouse serum.

Seo, B. et al (2020). Roseburia spp. Abundance Associates with Alcohol Consumption in Humans and Its Administration Ameliorates Alcoholic Fatty Liver in Mice. Cell host & microbe, 27(1), 25. Assay: alanine transaminase in mouse serum.

Jayaraman, S. et al (2020). Peptidase neurolysin functions to preserve the brain after ischemic stroke in male mice. Journal of neurochemistry, 153(1), 120. Assay: alanine transaminase in mouse plasma.

Abubakar, M. B. The Effects of Honey Administration on Petrol-Induced Oxidative Stress and Hepatotoxicity in Sprague-dawley Male Rats. Assay: alanine transaminase in mouse serum.

Durymanov, M., Permyakova, A., & Reineke, J. (2020). Pre-treatment With PLGA/Silibinin Nanoparticles Mitigates Dacarbazine-Induced Hepatotoxicity. Frontiers in Bioengineering and Biotechnology, 8. Assay: alanine transaminase in mouse serum.

Hay, Charles E., et al (2018). Development and testing of AAV-delivered single-chain variable fragments for the treatment of methamphetamine abuse. PloS one 13.6: e0200060. Assay: Alanine transaminase in mice serum.

Loeser, Konstantin, et al (2018). Protective effect of Casperome, an orally bioavailable frankincense extract, on lipopolysaccharide-induced systemic inflammation in mice. Frontiers in pharmacology 9: 387. Assay: Alanine transaminase in mice serum.

Shivange, Gururaj, et al (2018). A single-agent dual-specificity targeting of FOLR1 and DR5 as an effective strategy for ovarian cancer. Cancer cell 34.2: 331-345. Assay: Alanine transaminase in mice serum.

Akingbasote, James A., et al (2017). Improved hepatic physiology in hepatic cytochrome P450 reductase null (HRN) mice dosed orally with fenclozic acid. Toxicology research 6.1: 81-88. Assay: Alanine transaminase in mice plasma.

Kamerkar, Sushrut, et al (2017). Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer. Nature 546.7659: 498. Assay: Alanine transaminase in mice plasma.

Kim, Soo Hyun, et al (2017). Ezetimibe ameliorates steatohepatitis via AMP activated protein kinase-TFEB-mediated activation of autophagy and NLRP3 inflammasome inhibition. Autophagy 13.10: 1767-1781. Assay: Alanine transaminase in mice serum.

Seemann, Semjon, Franziska Zohles, and Amelie Lupp (2017). Comprehensive comparison of three different animal models for systemic inflammation. Journal of biomedical science 24.1: 60. Assay: Alanine transaminase in mouse serum.

Sharabi, K et al (2017). Selective chemical inhibition of PGC-1alpha gluconeogenic activity ameliorates type 2 diabetes. Cell 169.1: 148-160. Assay: Alanine transaminase in mice serum.

Vasquez, Kristine O., and Jeffrey D. Peterson (2017). Early detection of acute drug-induced liver injury in mice by noninvasive near-infrared fluorescence imaging. Journal of Pharmacology and Experimental Therapeutics 361.1: 87-98. Assay: Alanine transaminase in mice serum.

Wardah, J. Rahmahani, and T. Sopandi (2017). Effect of Phyllanthus buxifolius Leaf as a Feed Supplement on Liver Function and Haematological Response of Quail (Coturnix coturnix japonica) Challenged with Infectious Newcastle Disease Virus. International Journal of Poultry Science 16: 354-363. Assay: Alanine transaminase in quail serum.

Weis, Sebastian, et al (2017). Metabolic adaptation establishes disease tolerance to sepsis. Cell 169.7: 1263-1275. Assay: Alanine transaminase in mice plasma.

Akingbasote, James A., et al (2016). Hepatic effects of repeated oral administration of diclofenac to hepatic cytochrome P450 reductase null (HRN) and wild-type mice. Archives of toxicology 90.4: 853-862. Assay: Alanine transaminase in mice plasma.

Chaudhry, Kamaljit K., et al (2016). Glutamine supplementation attenuates ethanol-induced disruption of apical junctional complexes in colonic epithelium and ameliorates gut barrier dysfunction and fatty liver in mice. The Journal of nutritional biochemistry 27: 16-26. Assay: Alanine transaminase in mice plasma.

Chen, Yingqing, et al (2016). Synergistic effects of cilostazol and probucol on ER stress-induced hepatic steatosis via heme oxygenase-1-dependent activation of mitochondrial biogenesis. Oxidative medicine and cellular longevity 2016:3949813. Assay: Alanine transaminase in mice serum.

Mir, Hina, et al (2016). Occludin deficiency promotes ethanol-induced disruption of colonic epithelial junctions, gut barrier dysfunction and liver damage in mice. Biochimica et Biophysica Acta (BBA)-General Subjects 1860.4: 765-774. Assay: Alanine transaminase in mice plasma.

Seemann, Semjon, and Amelie Lupp (2016). Administration of AMD3100 in endotoxemia is associated with pro-inflammatory, pro-oxidative, and pro-apoptotic effects in vivo. Journal of biomedical science 23.1: 68. Assay: Alanine transaminase in mouse serum.

Wang, Huafeng, et al (2016). Adiponectin-derived active peptide ADP355 exerts anti-inflammatory and anti-fibrotic activities in thioacetamide-induced liver injury. Scientific reports 6: 19445. Assay: Alanine transaminase in mice serum.

Wang, Huafeng, et al (2016). Plumbagin protects liver against fulminant hepatic failure and chronic liver fibrosis via inhibiting inflammation and collagen production. Oncotarget 7.50: 82864. Assay: Alanine transaminase in mice serum.

Ballester-Lozano GF et al. (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr. 114(5):713-26. Assay: alanine aminotransferase enzyme activity in fish.

Dankel S, Degerud E et al (2014). Weight cycling promotes fat gain and altered clock gene expression in adipose tissue in C57BL/6J mice. Am J Physiol Endocrinol Metab. 15;306(2):E210-24. Assay: Alanine transaminase in mice plasma.

Peng M1, Xu S et al (2014). Thermosensitive Injectable Hydrogel Enhances the Antitumor Effect of Embelin in mouse Hepatocellular Carcinoma. J. Pharm. Sci., 103: 965-973. Assay: Alanine transaminase in mouse cell.

Kim HJ, Joe Y et al (2013). Carbon monoxide protects against hepatic ischemia/reperfusion injury via ROS-dependent Akt signaling and inhibition of glycogen synthase kinase 3beta. Oxidative Medicine and Cellular Longevity. 2013: 306421. Assay: Alanine transaminase in mice serum.

Zhang H, Xu H et al (2013). Inhibition of myeloperoxidase decreases vascular oxidative stress and increases vasodilatation in sickle cell disease mice. J Lipid Res. 54(11):3009-15. Assay: Alanine transaminase in mice plasma.

Mani V et al (2012) Effects of the Total Alkaloidal Extract of Murraya koenigii Leaf on Oxidative Stress and Cholinergic Transmission in Aged Mice. Phytotherapy Research. Assay: Alanine transaminase in mice serum.

rattan R et al (2011). Metformin suppresses ovarian cancer growth and metastasis with enhancement of cisplatin cytotoxicity in vivo. Neoplasia 13(5):483-491. Assay: Alanine transaminase in mouse plasma.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.