QuantiFluo™ Adenosine Deaminase Assay Kit

Protocol SDS

Application

• For quantitative determination of adenosine deaminase enzyme activity in biological samples.

Key Features

• Fast and Safe. The assay can be completed within 50 minutes—non-radioactive assay.
• Sensitive and accurate. The linear detection range is 0.3 to 30 U/L adenosine deaminase in a 96-well plate assay.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. It can be readily automated to assay thousands of samples per day.

Method

• FL415/475 nm

Samples

• Serum, saliva and other biological samples

Species

• All

Procedures

• 50 min

Size

• 100 tests in a 96-well plate

Detection Limit

• 0.3 U/L

Shelf Life

• 6 months

More Details

ADENOSINE DEAMINASE (EC 3.5.4.4) is a key enzyme in purine metabolism and plays an important role in the normal immune function of humans. ADA deficiency is one cause of Severe Combined Immunodeficiency (SCID), and elevated levels of ADA have been observed in patients with tuberculosis and immune-related diseases. BioAssay Systems’ DADA-100 Kit provides a convenient fluorimetric method to measure adenosine deaminase activity in biological samples. In this assay, liberated ammonia reacts with the reagents where the increase in fluorescence at λex/em = 415/475 nm is directly proportional to enzyme activity.

Is the assay compatible with primary amines?

This assay is compatible with most detergents, chelators and
buffer components. Protein concentrations up to 17 μg/mL are tolerated and
primary amine-containing buffers (e.g. Tris, glycine) should be kept below
10 mM, if possible. For best results, include the same concentration of the
sample buffer in the standards and blank

Does the kit contain Adenosine Deaminase?

No, the kit does not contain Adenosine Deaminase. The user must provide their own enzyme. The kit was optimized using calf intestine Adenosine Deaminase.

For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.