QuantiChrom™ Whole Blood Hemoglobin Kit

Protocol SDS

Application

• For quantitative determination of hemoglobin (Hb) in whole blood.

Key Features

• Safety. Non-hazardous. Reagents do not contain any toxic components, e.g. hexacyanoferrate(III) and potassium cyanide used in Drabkin’s reagent.
• Simple and high-throughput. The “mix-and-read” procedure involves the addition of a single reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
• Versatility. Assays can be executed in 96-well plates or cuvettes.
• High sensitivity. As low as 5 µL sample is used. Ideal when samples are limited such as mice blood samples.

Method

• OD570nm

Samples

• Whole blood samples

Species

• All

Procedure

• 5 min

Size

• 250 tests

Detection Limit

• 0.2 g/dL

Shelf Life

• 12 months

More Details

HEMOGLOBIN (Hb) is made of four globin chains each carrying a heme group. It is carried by red blood cells and transports oxygen from the lungs to the peripheral tissues to maintain the viability of cells. Quantitation of blood hemoglobin has been a key diagnostic parameter for various diseases such as anemia, polycythemia, and dehydration. Simple, direct, and automation-ready procedures for measuring hemoglobin concentration are becoming popular in Research and Drug Discovery. BioAssay Systems QuantiChrom™ Whole Blood Hb kit is based on an improved Triton/NaOH method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at 570 nm, is directly proportional to hemoglobin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Ward, E. J., Suleria, H. A. R., Joseph, M., Chambers, E., IV, Alavi, S., & Lindshield, B. L. (2020). Soy protein is an efficacious alternative to whey protein in sorghum-soy fortified blended foods in rats. Current Developments in Nutrition, 4(nzaa115). Assay: Whole Blood Hemoglobin Kit in Sprague Dawley rats blood.

Murata, Y., Yoshida, M., Sakamoto, N., Morimoto, S., Watanabe, T., & Namba, K. (2020). Iron uptake mediated by the plant-derived chelator nicotianamine in the small intestine. The Journal of Biological Chemistry. Assay: Whole Blood Hemoglobin Kit in mouse blood.

Ward, E. J. (2019). Efficacy of iron fortification and protein efficiency in fortified blended foods and extruded rice in rats [Thesis]. Assay: Whole Blood Hemoglobin Kit in weanling Sprague Dawley rats blood.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.