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EnzyChrom™ Uric Acid Assay Kit II



Application

  • Uric acid determination in biological samples such as cell culture media, biofluids (serum, urine), cell and tissue extracts.

Key Features

  • Sensitive. Use 10 μL samples. Linear detection range: Colorimetric assay 15 – 1000 μM Uric Acid. Fluorimetric assay 4 – 300 μM Uric Acid.
  • Fast. Run time is 30 minutes for Optical Density method and 10 minutes for Fluorimetric method for rapid results.
  • Convenient. Room temperature “mix-and-read” procedure can be readily automated for high-throughput assay of thousands of samples per day.

Method

  • OD570 or FL530/585nm

Samples

  • Biological samples such as cell culture media, biofluids (serum, urine), cell and tissue extracts.

Species

  • All

Procedures

  • 30 min

Size

  • 100 tests in 96-well plate

Detection Limit

  • 4 μM

Shelf Life

  • 6 months

More Details

URIC ACID is the breakdown product of purines such as adenosine and inosine. Humans and other primates are incapable of further metabolizing uric acid. In humans, uric acid is excreted via the kidneys, but a failure to remove excess uric acid can lead to conditions such as gout or uric acid kidney stones.

BioAssay Systems’ method provides a simple and high-throughput assay for measuring uric acid levels. In this assay, uric acid is enzymatically converted to allantoin, releasing H2O2. The resulting H2O2 reacts with a specific dye to form a pink-colored product. The change in OD570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to the uric acid present in the sample.

Can linear curve fitting be used to quantify uric acid?

Yes, at higher concentrations linear fitting can work, but in most cases, hyperbolic curve fitting provides the most accurate results due to the enzymatic nature of this assay.

Can I use Microsoft Excel or Google Sheets to do the hyperbolic curve fitting to determine the uric acid quantification?

Unfortunately, no. Neither Microsoft Excel nor Google Sheets has the ability to do hyperbolic curve fitting. To obtain a hyperbolic equation (y = (m × x)/(k + x)), the standard curve values must be exported to an application such as GraphPad or an online curve fitting tool such as mycurvefit.com. Once you have the curve equation, you can employ What-If Analysis and the Goal Seek function to solve for your unknown concentrations.

Is there a way to increase the sensitivity of the assay?

Yes, the sample volume could be increased to anywhere from 20-40 μL. The volumes of the standards will need to be increased by the same volume. In all cases, the volume of the Working Reagent needs to be greater than the volume of the sample.

For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.

$439.00
- +

For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.

Orders are shipped the same day if placed by 2pm PST
Shipping: Ice
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.

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