EnzyChrom™ Superoxide Dismutase Assay Kit

Protocol SDS

Application

• For quantitative determination of superoxide dismutase (SOD) enzyme activity and evaluation of drug effects.

Key Features

• Sensitive and accurate. Linear detection range of 0.05 – 3 U/mL SOD.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD440nm

Samples

• Blood, cell, tissue, etc

Species

• All

Procedure

• 1 hr

Size

• 100 tests

Detection Limit

• 0.05 U/mL

Shelf Life

• 6 months

More Details

Superoxide dismutases (SOD, EC1.15.1.1) are enzymes that catalyze the dismutation of superoxide into O₂ and H₂O₂. They are an important antioxidant defense in all cells exposed to O2. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders, and cancer. BioAssay Systems SOD assay provides a convenient colorimetric means for the quantitative determination of SOD enzyme activity in biological samples. In the assay, superoxide (O₂^–) is provided by a xanthine oxidase (XO) catalyzed reaction. O₂^– reacts with a WST-1 dye to form a colored product. SOD scavenges the O₂^– thus less O₂^– is available for the chromogenic reaction. The color intensity (OD440nm) is used to determine the SOD activity in a sample.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Humam, A. M., et al. (2021). Supplementation of postbiotic RI11 improves antioxidant enzyme activity, upregulated gut barrier genes, and reduced cytokine, acute phase protein, and heat shock protein 70 gene expression levels in heat-stressed broilers. Poultry Science. 100(3): 100908. Assay: Superoxide Dismutase in chicken plasma.

Shukla, M., et al. (2020). Moderate noise associated oxidative stress with concomitant memory impairment, neuro-inflammation and neurodegeneration. Brain, Behavior, & Immunity – Health. 5: 100089. Assay: Superoxide Dismutase in rat hippocampus tissue.

Patang, F., Soegianto, A. (2020). Oxidative responses of macro-invertebrates in relation to environmental variables in rivers of East Kalimantan, Indonesia. Chemistry and Ecology. 36(9): 855-867. Assay: Superoxide Dismutase in Chironominae, Gomphus, and Lestes larvae.

Magana-Cerino, J. M., et al. (2020). Consumption of nixtamal from a new variety of hybrid blue maize ameliorates liver oxidative stress and inflammation in a high-fat diet rat model. Journal of Functional Foods. 72: 104075. Assay: Superoxide Dismutase in rat liver tissue.

Nawi, A., et al. (2020). Lipid peroxidation in the descending thoracic aorta of rats deprived of REM sleep using the inverted flowerpot technique. Experimental Physiology. 105(8): 1223-1231. Assay: Superoxide Dismutase in rat thoracic aorta.

AlAsmari, A. F., et al. (2020). Liraglutide attenuates gefitinib-induced cardiotoxicity and promotes cardioprotection through the regulation of MAPK/NF-κB signaling pathways. Saudi Pharmaceutical Journal. 28(4): 509-518. Assay: Superoxide Dismutase in rat heart tissue.

Wahyuningsih, S. P. A., et al. (2020). Antioxidant and nephroprotective effects of okra pods extract (abelmoschus esculentus l.) against lead acetate-induced toxicity in mice. Scientifica. 2020: 1-10. Assay: Superoxide Dismutase in mouse serum.

Sahin, T. D., et al. (2020). Resveratrol and quercetin attenuate depressive-like behavior and restore impaired contractility of vas deferens in chronic stress-exposed rats: Involvement of oxidative stress and inflammation. Naunyn-Schmiedeberg ‘s Archives of Pharmacology. 393(5): 761-775. Assay: Superoxide Dismutase in rat tissue.

Sahin, T. D., et al. (2020). Infliximab prevents dysfunction of the vas deferens by suppressing inflammation and oxidative stress in rats with chronic stress. Life Sciences. 250: 117545. Assay: Superoxide Dismutase in rat tissue.

Humam, A. M., et al. (2020). Dietary supplementation of postbiotics mitigates adverse impacts of heat stress on antioxidant enzyme activity, total antioxidant, lipid peroxidation, physiological stress indicators, lipid profile and meat quality in broilers. Animals: An Open Access Journal from MDPI. 10(6). Assay: Superoxide Dismutase in chicken plasma.

Qi, J.,et al. (2020). Efficient Microcystis aeruginosa removal by moderate photocatalysis-enhanced coagulation with magnetic Zn-doped Fe3O4 particles. Water Research. 171: 115448. Assay: Superoxide Dismutase in Microcystis aeruginosa cells.

Wee, C. L., et al. (2021). Calcitriol supplementation ameliorates microvascular endothelial dysfunction in vitamin d-deficient diabetic rats by upregulating the vascular enos protein expression and reducing oxidative stress. Oxidative Medicine and Cellular Longevity. 2021. 3109294. Assay: Superoxide Dismutase in rat tissue.

Xin, X., et al. (2020). Exploring the biophysicochemical alteration of green alga Asterococcus superbus interactively affected by nanoparticles, triclosan and illumination. Journal of Hazardous Materials. 398: 122855. Assay: Superoxide Dismutase in Asterococcus superbus cells.

Pena-Bermudez, Y. A., et al. (2020). Effects of feeding increasing levels of yerba mate on lamb meat quality and antioxidant activity. Animals. 10(9): 1458. Assay: Superoxide Dismutase in lamb meat.

Mukherjee, S., et al. (2020). Sterol 14-α-demethylase is vital for mitochondrial functions and stress tolerance in Leishmania major. PLOS Pathogens. 16(8): e1008810. Assay: Superoxide Dismutase in Leishmania amazonensis promastigotes.

Alqarni, M. M., Osman, M. A., Al-Tamimi, D. S., Gassem, M. A., Al-Khalifa, A. S., Al-Juhaimi, F., & Mohamed Ahmed, I. A. (2019). Antioxidant and antihyperlipidemic effects of Ajwa date (Phoenix dactylifera L.) extracts in rats fed a cholesterol-rich diet. Journal of Food Biochemistry, e12933. Assay: Superoxide dismutase in mouse.

Cheong, A. M., Patrick, N. O., Tan, C. P., Lim, Y. M., & Nyam, K. L. (2018). Improvement of gastroprotective and anti-ulcer effect of kenaf seed oil-in-water nanoemulsions in rats. Food science and biotechnology, 27(4), 1175-1184. Assay: Superoxide dismutase in rat stomach tissue.

Gonzalez Gomez, M. L. (2018). Methylglyoxal Effects in Cell Therapy for Myocardial Infarction (Doctoral dissertation, Universite d’Ottawa/University of Ottawa). Assay: Superoxide dismutase in bacteria BMC cells.

Jimenez, S.R. (2018). Evaluation of Dietary Phytochemicals as Rumen Modifiers in Lactating Dairy Cows. Electronic Theses and Dissertations. 2647. Assay: Superoxide dismutase in rumen.

Paul, S., Gangwar, A., Bhargava, K., & Ahmad, Y. (2018). STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient. Redox biology, 14, 423-438. Assay: Superoxide dismutase in rat lung tissue.

Wongmekiat, O., Peerapanyasut, W., & Kobroob, A. (2018). Catechin supplementation prevents kidney damage in rats repeatedly exposed to cadmium through mitochondrial protection. Naunyn-Schmiedeberg’s archives of pharmacology, 391(4), 385-394. Assay: Superoxide dismutase in rat serum.

Oliveira, C. S., Rodrigues, A. M., Nogueira, G. B., Nascimento, M. A., Punaro, G. R., & Higa, E. M. S. (2017). Moderate aerobic exercise on the recovery phase of gentamicin-induced acute kidney injury in rats. Life sciences, 169, 37-42. Assay: Superoxide dismutase in rat kidney tissue.

Paul, S., Gangwar, A., Bhargava, K., & Ahmad, Y. (2017). Deciphering Molecular Cascades In A Novel Acclimatization Strategy For Rapid Ascent To High Altitude. bioRxiv, 145342. Assay: Superoxide dismutase in rat lung tissue.

Sayin, O., Micili, S. C., Goker, A., Kamaci, G., Ergur, B. U., Yilmaz, O., & Akdogan, G. G. (2017). The role of resveratrol on full-Thickness uterine wound healing in rats. Taiwanese Journal of Obstetrics and Gynecology, 56(5), 657-663. Assay: Superoxide dismutase in rat uterine tissue.

Seyedan, A., Alshawsh, M. A., Alshagga, M. A., & Mohamed, Z. (2017). Antiobesity and lipid lowering effects of Orthosiphon stamineus in high-fat diet-induced obese mice. Planta medica, 83(08), 684-692. Assay: Superoxide dismutase in rat liver cell supernatant.

Shenai-Tirodkar, P. S., Gauns, M. U., Mujawar, M. W. A., & Ansari, Z. A. (2017). Antioxidant responses in gills and digestive gland of oyster Crassostrea madrasensis (Preston) under lead exposure. Ecotoxicology and environmental safety, 142, 87-94. Assay: Superoxide dismutase in oyster gills and digestive glands.

Ahmad, L. M., Al-Ismail, K. M., & Yamani, M. I. (2016). Influence of processing and storage on chemical and biochemical characteristics of Mish cheese traditionally produced in Jordan. Quality Assurance and Safety of Crops & Foods, 8(2), 243-248. Assay: Superoxide dismutase in cheese.

Arjinajarn, P., Pongchaidecha, A., Chueakula, N., Jaikumkao, K., Chatsudthipong, V., Mahatheeranont, S., & Lungkaphin, A. (2016). Riceberry bran extract prevents renal dysfunction and impaired renal organic anion transporter 3 (Oat3) function by modulating the PKC/Nrf2 pathway in gentamicin-induced nephrotoxicity in rats. Phytomedicine, 23(14), 1753-1763. Assay: Superoxide dismutase in rat renal cortical tissue.

Ismiyati, A et al. (2016). Protective Effect of Vitamins C and E on Depot-Medroxyprogesterone Acetate-Induced Ovarian Oxidative Stress In Vivo. J Toxicol. 2016:3134105. Assay: Superoxide dismutase in rat ovarium tissue.

Jaikumkao, K., Pongchaidecha, A., Chattipakorn, N., Chatsudthipong, V., Promsan, S., Arjinajarn, P., & Lungkaphin, A. (2016). Atorvastatin improves renal organic anion transporter 3 and renal function in gentamicin-induced nephrotoxicity in rats. Experimental physiology, 101(6), 743-753. Assay: Superoxide dismutase in rat renal cortical tissue.

Paul, S., Arya, A., Gangwar, A., Bhargava, K., & Ahmad, Y. (2016). Size restricted silymarin suspension evokes integrated adaptive response against acute hypoxia exposure in rat lung. Free Radical Biology and Medicine, 96, 139-151. Assay: Superoxide dismutase in rat lung tissue.

Poontawee, W., Natakankitkul, S., & Wongmekiat, O. (2016). Protective effect of Cleistocalyx nervosum var. paniala fruit extract against oxidative renal damage caused by cadmium. Molecules, 21(2), 133. Assay: Superoxide dismutase in rat kidney tissue.

Santharaman, P., Das, M., Singh, S. K., Sethy, N. K., Bhargava, K., Claussen, J. C., & Karunakaran, C. (2016). Label-free electrochemical immunosensor for the rapid and sensitive detection of the oxidative stress marker superoxide dismutase 1 at the point-of-care. Sensors and Actuators B: Chemical, 236, 546-553. Assay: Superoxide dismutase in human keratinocytes.

Ahmad, LM et al (2015). Influence of processing and storage on chemical and biochemical characteristics of Mish cheese traditionally produced in Jordan. Quality Assurance and Safety of Crops & Foods: 8(2):243-248. Assay: Superoxide Dismutase in Cheese.

Arya, A et al. (2015). NAP (davunetide) protects primary hippocampus culture by modulating expression profile of antioxidant genes during limiting oxygen conditions. Free Radic Res.49(4):440-52. Assay: Superoxide dismutase in rat cells.

Kusurkar, TS et al (2015). A glowing antioxidant from tasar silk cocoon. (126): 04563-104573. Assay: Superoxide dismutase in moth.

Laili, AN et al. (2015). Protective effect of combined vitamin C and E against ovarian and endometrial toxicity in rats that receiving oral rhodamine B. Biomarkers and Genomic Medicine. 7(4):154-158. Assay: Superoxide dismutase in rat ovarium tissue.

Veri, N et al. (2015). Protective effect of green tea against ovarian and endometrial apoptoses in rats treated with depot medroxyprogesterone acetate. Biomarkers and Genomic Medicine. 7(3): 105-109. Assay: Superoxide dismutase in rat green tea extract.

Abdel-Latif, HMR and Khalil, RH (2014). Evaluation of two Phytobiotics, Spirulina platensis and Origanum vulgare extract on Growth, Serum antioxidant activities and Resistance of Nile tilapia (Oreochromis niloticus) to pathogenic Vibrio alginolyticus. Intern. J. Fisheries and Aquatic Studies 1(5): 250-255. Assay: Superoxide dismutase in fish plasma.

Abdel-Latif, HMR and Khalil, RH (2014). Evaluation of two Phytobiotics, Spirulina platensis and Origanum vulgare extract on Growth, Serum antioxidant activities and Resistance of Nile tilapia (Oreochromis niloticus) to pathogenic Vibrio alginolyticus. Intern. J. Fisheries and Aquatic Studies 1(5): 250-255. Assay: Superoxide Dismutase in fish plasma.

Dura, R et al. (2014). Oxidative stress indicators in patients with prostate disorders in Enugu, South-East Nigeria. Biomed Res Int. 2014:313015. Assay: Superoxide Dismutase in human cells.

Duru, R et al. (2014). Oxidative stress indicators in patients with prostate disorders in Enugu, South-East Nigeria. Biomed Res Int. 2014:313015. Assay: Superoxide dismutase in human cells.

Mohamed A et al. (2014). Hemin Attenuates Cisplatin-Induced Acute Renal Injury in Male rats.Oxid Med Cell Longev. 2014:476430. Assay: Superoxide dismutase in rat kidney tissue.

Mohammedi, K et al. (2014). Manganese superoxide dismutase (SOD2) polymorphisms, plasma advanced oxidation protein products (AOPP) concentration and risk of kidney complications in subjects with type 1 diabetes. PLoS One.9(5):e96916. Assay: Superoxide dismutase in human plasma.

Struchtemeyer, SG et al. (2014). Survival of the anaerobic fungus Orpinomyces sp. strain C1A after prolonged air exposure. Sci Rep. 4: 6892. Assay: Superoxide dismutase in angus steer protein.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

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