EnzyChrom™ Sphingomyelin Assay Kit
- For quantitative determination of sphingomyelin in biological samples (serum, plasma, etc.).
- Safe. Non-radioactive assay.
- Sensitive and accurate. Linear detection range in a 96-well plate: 1 – 50 mg/dL sphingomyelin for colorimetric assays and 0.07 – 7 mg/dL sphingomyelin for fluorimetric assays.
- Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.
- OD570nm or FL530/585nm
- Serum, plasma, and other biological samples
- 30 min
- 100 tests in 96-well plate
- OD, FL: 1, 0.07 mg/dL
- 6 months
More DetailsSPHINGOMYELINS (ceramide phosphocholine, SM) are key lipid components of cell membranes and lipoproteins. Sphingomyelinases (SMases) hydrolyze most sphingomyelins into ceramide and phosphocholine. This release of ceramide contributes to cell signaling pathways resulting in cell proliferation, differentiation, growth arrest, and apoptosis. Niemann-Pick disease is identified by the accumulation of sphingomyelin due to deficiencies in sphingomyelinase activity. Increased sphingomyelin levels have also been observed in several diseases, such as atherosclerosis, so simple, direct, and automation-ready procedures for measuring sphingomyelins are very desirable. BioAssay Systems’ EnzyChrom™ Sphingomyelin Assay Kit provides a convenient colorimetric and fluorimetric method to quantify sphingomyelin in biological samples. In this assay, sphingomyelin is enzymatically converted to hydrogen peroxide, which forms a colored product with the dye reagent that is measurable at OD570 nm or fluorescence at λex/em = 530/585nm. The colored product is directly proportional to the sphingomyelin concentration in the sample.
Does this kit measure all sphingomyelins?
This kit measures all sphingomyelins that are made up of phosphocholine and ceramide, but will not measure sphingomyelins with a phosphoethanolamine head.
I am noticing a precipitate in Enzyme Mix when reconstituted. Is this normal and will it affect the results?
It is not uncommon for precipitate to appear in Enzyme Mix. If precipitate is observed, centrifuge for 2 min at 14,000 rpm and use the clear supernatant to run the assay. This should not affect results.
For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.
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