QuantiChrom™ Pyrophosphatase Inhibitor Assay Kit

Protocol SDS

Application

• For high-throughput inhibitor screening and evaluation of pyrophosphatase modulators.

Key Features

• Safe. Non-radioactive assay.
• Fast and convenient. The procedure involves incubation of enzyme and inhibitor, addition of a single working reagent and incubation for 30 min. Room temperature assay. No 37°C incubator is needed.
• High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems. Robust assay with a Z’ factor of 0.59. Can be used in 96-well, 384-well, and potentially higher density screening assays.

Method

• OD620

Samples

• Compounds that affect pyrophosphatase activity

Species

• All

Procedures

• 70 min

Size

• 100 tests in 96well plate

Detection Limit

• NA

Shelf Life

• 6 months

Screening Services

• BioAssay Systems offers comprehensive Pyrophosphatase lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us by Service.

More Details

INORGANIC PYROPHOSPHATASE(E.C.3.6.1.1) catalyzes the hydrolysis of phosphoester bonds of inorganic pyrophosphate [P₂O₇^4-], thereby releasing two orthophosphate molecules. Family I PPases are essential enzymes found in all kingdoms of life and are responsible for maintaining the correct pyrophosphate equilibrium necessary to carry out nucleic acid and protein synthesis, and facilitate fatty acid beta oxidation.