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QuantiChrom™ Peroxide Assay Kit II
Application
- Direct Assay of H2O2 and organic peroxides in biological samples (e.g. serum, citrate-plasma, urine, cell lysate, culture medium) and the determination of the effects of drugs on peroxide metabolism.
Key Features
- Sensitive and accurate. Detection limit of 0.54 µM (18.4 ng/mL) and linear up to 30 µM (1,020 ng/mL) H2O2 in 96-well plate assay.
- Simple and high-throughput. The procedure involves addition of a single, ready-to-use detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
Method
- OD585nm
Samples
- Biological samples (e.g. serum, citrate-plasma, urine, cell lysate, culture medium).
Species
- All
Procedures
- 30 min
Size
- 100 tests in 96-well plate
Detection Limit
- 0.54 µM
Shelf Life
- 6 months
More Details
Peroxide (e.g. hydrogen peroxide H2O2) is one of the key reactive oxygen species formed under oxidative stress conditions. High levels of peroxide formation have been linked to pathological conditions such as aging, asthma, diabetes, atherosclerosis, cataracts, inflammatory arthritis and neurodegenerative diseases.Simple, direct and automation-ready procedures for quantitative determination of peroxide find wide applications in research and drug discovery. BioAssay Systems’ improved peroxide assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes the chromogenic Fe3+-xylenol orange reaction, in which a purple complex is formed when Fe2+ provided in the reagent is oxidized to Fe3+ by peroxides present in the sample. The intensity of the color, measured at 540-610nm, is an accurate measure of the peroxide level in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
$399.00
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Delivery: 2-6 days (US), 6-15 days (intl)
Storage: -20°C upon receipt.
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