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EnzyFluo™ MMP-9 Inhibitor Assay Kit
Application
- For evaluation of drugs and screening of potential inhibitors to MMP-9.
Key Features
- Robust. Fluorescence-based assay with a longer wavelength probe (λex/em = 490/520 nm), minimizing test compound interference. Robust assay with a Z’ factor of > 0.8.
- Homogeneous and convenient. Homogenous “mix-incubate-measure” assay. No wash and reagent transfer steps are involved.
- High-throughput. Can be readily automated to assay thousands of samples per day.
Method
- FL490/520nm
Samples
- Compounds that affect MMP-9 activity.
Species
- Human
Procedures
- 75 min
Size
- 100 tests in 96-well plate.
Shelf Life
- 6 months
Screening Services
- BioAssay Systems offers comprehensive MMP-9 lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us by Service.
More Details
MATRIX METALLOPROTEINASE-9 or MMP-9 (EC 3.4.24.35) is a Ca2+-and Zn2+-dependent, interstitial gelatinase that plays a role in the remodeling of the extracellular matrix of tissues. MMP-9 plays a central role in processes diverse as wound healing, angiogenesis, and bone development. In addition to its role in normal growth and development, MMP-9 is up-regulated in many diseases, including metastatic cancer, rheumatoid arthritis, and asthma. BioAssay Systems’ EnzyFluo™ MMP-9 Inhibitor Assay Kit uses a quenched synthetic substrate. Upon digestion by MMP-9, the substrate is cleaved, which abolishes the quenching of the fluorescence label. The fluorescent fragment is measured at λex/em = 490/520 nm. Inhibition is determined by the decrease in fluorescence.Does the MMP-9 in the kit need to be activated by either APMA or Trypsin?
No activation steps are required. We have already treated the MMP-9 to activate it.
Is it required to run this assay at 30°C?
Not necessarily. While the kit was developed at 30°C, it can be run at room termperature, but expect that the reaction velocity will be lower. You may also run this reaction at 37°C, in which case the reaction velocity will be greater.
What is the MMP-9 enzyme used in the kit?
The MMP-9 enzyme is a recombinant human protein expressed in HEK293 cells with AA A20-E707 . The MMP-9 is roughly a 90 kDa protein due to posttranslation glycosylation.
For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
$629.00
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: Ice
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.
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