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EnzyFluo™ MMP-1 Inhibitor Assay Kit
Application
- For evaluation of drugs and screening of potential inhibitors to MMP-1.
Key Features
- Robust. Fluorescence-based assay with a longer wavelength probe (λex/em = 490/520 nm), minimizing test compound interference. Robust assay with a Z’ factor of > 0.8.
- Homogeneous and convenient. Homogenous “mix-incubate-measure” assay. No wash and reagent transfer steps are involved.
- High-throughput. Can be readily automated to assay thousands of samples per day.
Method
- FL490/520 nm
Samples
- Compounds that affect MMP-1.
Species
- Human
Procedures
- 75 min
Size
- 100 tests (Custom bulk sizes available upon request)
Shelf Life
- 6 months
Screening Services
- BioAssay Systems offers comprehensive MMP-1 lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us by Service.
More Details
MATRIX METALLOPROTEINASE-1 or MMP-1 (EC 3.4.24.7) is a Ca2+– and Zn2+-dependent, interstitial collagenase that plays a role in the remodeling of the extracellular matrix of tissues. MMP-1 is up-regulated in metastatic cancer cells and in rheumatoid arthritis.BioAssay Systems’ EnzyFluo™ MMP-1 Inhibitor Assay Kit uses a quenched synthetic substrate. Upon digestion by MMP-1, the substrate is cleaved, which abolishes the quenching of the fluorescence label. The fluorescent fragment is measured at λex/em = 490/520 nm. Inhibition is determined by the decrease in fluorescence.Does the MMP-1 in the kit need to be activated by either APMA or Trypsin?
No, the MMP-1 enzyme included in the kit does not require an activation step prior to use.
Is it required to run this assay at 30°C?
Not necessarily. While the kit was developed at 30°C, it can be run at room termperature, but expect that the reaction velocity will be lower. You may also run this reaction at 37°C, in which case the reaction velocity will be greater.
What is the MMP-1 enzyme used in the kit?
The MMP-1 enzyme is a recombinant human protein expressed in E. coli. The MMP-1 is a 42.7 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain, which is involved in substrate specificity, and in binding TIMP-1.
For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
$629.00
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: Ice
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.
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