QuantiChrom™ α-L-Fucosidase Assay Kit

Protocol SDS

Application

• For quantitative determination of α-L-Fucosidase activity in biological samples.

Key Features

• High sensitivity and wide linear range. Linear detection range (10 µL sample): 1 to 100 U/L.
• Homogeneous and simple procedure. A simple add-mix-read procedure allows reliable quantitation of fucosidase activity within 20 minutes.
• Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Can be readily automated to measure thousands of samples per day.

Method

• OD405nm

Samples

• Plasma, serum, tissue, cell lysate, etc

Species

• All

Procedure

• 20 min

Size

• 100 tests

Detection Limit

• 1 U/L

Shelf Life

• 6 months

More Details

α-L-FUCOSIDASE (AFU) is an enzyme coded by the FUCA1 gene in humans and catalyzes the breakdown of L-Fucose. A genetic deficiency in this enzyme results in a neurovisceral storage disease, fucosidosis, which is characterized by the accumulation of fucose. Low serum activity of fucosidase has also been linked to ovarian carcinoma. Elevated fucosidase serum activity has been observed in patients with diabetes, hyperthyroidism, cirrhosis, and hepatitis. Increased activity has been associated with lung, breast, stomach, ovary, uterus, and liver carcinomas.BioAssay System’s non-radioactive, colorimetric AFU assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after the addition of the stop reagent. The increase in absorbance at 405 nm after the addition of the stop reagent is directly proportional to the enzyme activity.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.