EnzyChrom™ L-Arginine Assay Kit

Protocol SDS

Application

• For quantitative determination of L-arginine in food, beverage, biological samples (e.g. serum, cell lysate, etc).

Key Features

• Simple. Using our internal standard method and robust reagents, no sophisticated sample pretreatment is required.
• Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 0.066 to 3 mM for colorimetric assays.
• High-throughput. Can be readily automated to assay thousands of samples per day.

Method

• OD450nm

Samples

• Food, beverage, and biological samples (e.g. serum, cell lysate, etc)

Species

• All

Procedures

• 90 min

Size

• 100 tests in 96-well plate

Detection Limit

• 0.066 mM

Shelf Life

• 6 months

More Details

L-ARGININE is an amino acid essential for protein metabolism and primarily found in the active sites of various proteins. During nitrogen metabolism, arginase breaks down arginine into urea and ornithine. L-Arginine is also present in many protein-rich foods and beverages. In particular, its detection in beverages, such as juices and wine, is crucial for quality control.

BioAssay Systems' L-Arginine assay measures the urea generated from arginase hydrolysis of arginine with our proprietary reagents. The increase in absorbance at 450 nm is proportional to the arginine concentration.

What is the normal concentration of arginine in blood?

A normal range of arginine in the blood is 21-150 μM. For patients with argininemia, the arginine concentration is typically 3-4 times higher than normal.

My samples became turbid after adding the urea reagent. How can I fix it?

For some samples, addition of urea reagent may cause turbidity. Test several dilutions to determine the optimal sample dilution factor at which turbidity does not occur. If it still occurs, transfer sample to an Eppendorf tube and centrifuge for 5 minutes at 14,000 rpm. Transfer supernatant back to reaction plate and read the absorbance.

What factors could potentially interfere with this assay?

Low pH of the sample could interfere with this assay since the pH optimum of arginase is between pH 9.0 and 10.0. Beverage samples with low pH (e.g. fruit juices) must be neutralized with 6N NaOH before running the assay.

For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.