EnzyChrom™ HDL and LDL/VLDL Assay Kit

Protocol SDS

Application

• For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.

Key Features

• Sensitive and accurate. Requires only 20 µL serum sample. The detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay.
• Convenient. Room temperature assay. No 37°C heater is needed.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD340nm

Samples

• Serum

Species

• All

Procedure

• 60 min

Size

• 100 tests

Detection Limit

• 5 mg/dL

Shelf Life

• 6 months

More Details

Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors of coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to a higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined but can be changed by medications, food choices, and other factors. Simple, direct, and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. BioAssay Systems HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using cholesterol esterase/cholesterol dehydrogenase reagent. In this reaction, NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample.

Would you please confirm me cat# EHDL-100 can be used for Rat or not?

Yes, the assay is not species-specific and works with rat serum samples.

Could they be used with lung tissue homogenates?

Yes, but the EnzyChrom™ HDL and LDL/VLDL assay kits were developed for serum samples to detect the various transport forms of cholesterol. For tissue samples I would rather recommend using our cholesterol assay kits ECCH-100/E2CH-100.

We would like to know if, during the procedure and before the OD measurement, the reagent added to the serum inactivates hepathitis C virus.

No. The EHDL-100 assay reagents will not inactivate hepatitis C virus, or any other virus.

Lu, H et al. (2021). Hepatocyte nuclear factor 4a and glucocorticoid receptor coordinately regulate lipid metabolism in mice fed a high-fat-high-sugar diet. BioRxiv, 2021.02.06.427306. Assay: HDL/LDL in mouse serum.

Poulsen, SS et al. (2021). A transcriptomic overview of lung and liver changes one day after pulmonary exposure to graphene and graphene oxide. Toxicology and Applied Pharmacology, 410, 115343. Assay: HDL/LDL in mouse plasma.

Kim, J et al. (2020). Effects of exercise training and detraining on atheromatous matrix metalloproteinase activity in mice. Atherosclerosis, 299: 15-23. Assay: HDL/LDL in mouse serum.

Mizelman, E et al. (2020). A low-glycemic index, high-fiber, pulse-based diet improves lipid profile, but does not affect performance in soccer players. Nutrients, 12(5), 1324. Assay: HDL/LDL in human serum.

Sudirman, S et al. (2019). A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese rats. Food & Function, 10(9): 5697-5706. Assay: HDL/LDL in rat serum.

Das, A et al. (2019). Novel interaction of antioxidant-1 with TRAF4: Role in inflammatory responses in endothelial cells. American Journal of Physiology. Cell Physiology, 317(6): C1161-C1171. Assay: HDL/LDL in mouse plasma.

Jager, M., Hubert, A., Gogiraju, R., Bochenek, M. L., Munzel, T., & Schafer, K. (2019). Inducible knockdown of endothelial protein tyrosine phosphatase-1B promotes neointima formation in obese mice by enhancing endothelial senescence. Antioxidants & redox signaling, 30(7), 927-944. Assay: HDL and LDL/VLDL in mice serum.

Sharma, V., Smolin, J., Nayak, J., Ayala, J. E., Scott, D. A., Peterson, S. N., & Freeze, H. H. (2018). Mannose Alters Gut Microbiome, Prevents Diet-Induced Obesity, and Improves Host Metabolism. Cell reports, 24(12), 3087-3098. Assay: HDL and LDL/VLDL in mice serum.

Bergt, S., Grub, A., Wagner, S., Engelke, H., Noldge-Schomburg, G., Vollmar, B. & Wagner, N. M. (2017). Pravastatin but not simvastatin improves survival and neurofunctional outcome after cardiac arrest and cardiopulmonary resuscitation. JACC: Basic to Translational Science, 2(2), 149-159. Assay: HDL and LDL/VLDL in mice plasma.

Shon, S. M., Jang, H. J., Schellingerhout, D., Kim, J. Y., Ryu, W. S., Lee, S. K. & Je, K. H. (2017). Cytokine Response to Diet and Exercise Affects Atheromatous Matrix Metalloproteinase-2/9 Activity in Mice. Circulation Journal 81(10):1528-1536. Assay: HDL and LDL/VLDL in mice serum.

Stanton, Catherine, et al (2017). Exopolysaccharide-producing bacteria, and uses thereof for protecting heart health. U.S. Patent No. 9,808,493 Assay: HDL and LDL/VLDL in mice serum.

Frej, C., Linder, A., Happonen, K. E., Taylor, F. B., Lupu, F., & Dahlback, B. (2016). Sphingosine 1-phosphate and its carrier apolipoprotein M in human sepsis and in Escherichia coli sepsis in baboons. Journal of cellular and molecular medicine, 20(6), 1170-1181. Assay: HDL and LDL/VLDL in baboon plasma.

Ballester-Lozano GF et al. (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr. 114(5):713-26. Assay: HDL and LDL/VLDL in fish.

Bourdon JA et al (2012) Hepatic and pulmonary toxicogenomic profiles in mice intratracheally instilled with carbon black nanoparticles reveal pulmonary inflammation, acute phase response, and alterations in lipid homeostasis. Toxicol Sci 127(2):474-84. Assay: HDLandLDL/VLDL in mice plasma.

Shanmugasundaram R & Selvaraj RK (2011). Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr(Berl) 95(6):762-70. Assay: HDL/LDL in mouse blood.

Shon, SM et al (2011). Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque. Atherosclerosis 216 (1): 67-73. Assay: HDLandLDL/VLDL in rat serum.

Shon, S-M. et al. (2011). Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque. Atherosclerosis 216 (1): 67-73. Assay: HDL and LDL/VLDL in mouse blood.

Uddin MJ et al (2011) Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genet 12:62. Assay: HDLandLDL/VLDL in pig serum.

Oliver SR et al (2010). Increased oxidative stress and altered substrate metabolism in obese children. Int J Pediatr Obes. 2010 Oct;5(5):436-44. Assay: HDL/LDL in bird serum.

Ponda MP, et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis. 210(1):57-62. Assay: HDLandLDL/VLDL in mouse Blood.

Ponda, MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210 (1): 57-62. Assay: HDL/LDL in human plasma.

Shanmugasundaram R, Selvaraj RK (2010). Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr (Berl). [Epub ahead of print]. Assay: HDLandLDL/VLDL in bird serum.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.