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EnzyChrom™ AF HDL and LDL/VLDL Assay Kit


  • For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.

Key Features

  • Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays.
  • Convenient. Room temperature assay. No 37°C heater is needed.


  • OD570nm, or FL530/585nm


  • Serum


  • All


  • 60 min


  • 100 tests

Detection Limit

  • OD, FL: 1, 0.2 mg/dL

Shelf Life

  • 6 months

More Details

CHOLESTEROL concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. BioAssay Systems HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to total cholesterol concentration in the sample.

I want to ask about the differences between “EnzyChrom™ AF HDL and LDL/VLDL Assay Kit and EnzyChrom™ HDL and LDL/VLDL Assay Kit. Since I want to work on mice.

Both kits will work with mouse serum and plasma samples, but I would recommend using serum, because we have not validated the use of plasma. The EHDL-100 kit has a linear detection range of 5 -300 mg/dL. It requires a microplate reader with a 340nm filter. The E2HL-100 kit is more sensitive with a linear detection range of 1 -100 mg/dL using the colorimetric readout (requires a 570 nm filter), or 0.2 -10 mg/dL with the fluorimetric readout (requires a fluorescence microplate reader).

Colijn, S et al. (2020). Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis. Disease Models & Mechanisms, 13(1). Assay: HDL/LDL in mouse plasma.

Lambert, K et al. (2020). Biocompatible modified water as a non-pharmaceutical approach to prevent metabolic syndrome features in obesogenic diet-fed mice. Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research Association, 141, 111403. Assay: HDL/LDL in mouse serum and liver.

Maciak, S et al. (2020). Low basal metabolic rate as a risk factor for development of insulin resistance and type 2 diabetes. BMJ Open Diabetes Research & Care, 8(1). Assay: HDL/LDL in mouse plasma.

Feng, J., Li, L., Ou, Z., Li, Q., Gong, B., Zhao, Z. & Yang, X. (2018). IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity. Cellular & molecular immunology, 15(5), 493. Assay: HDL and LDL/VLDL in mice tissue/plasma.

Heriansyah, T., Lutfiana, N. C., Sulfia, Y. H., Sholichah, Z., Lestari, R. D., Sargowo, D., & Wihastuti, T. A. (2018). Inhibition of Adhesion Molecule Expression in Early Progression of Atherosclerosis in Dyslipidemia Model on Sprague Dawley rats by Darapladib. International Journal of Pharmacology, 14(3), 444-450. Assay: HDL and LDL/VLDL in sprague dewley rats serum.

Heriansyah, T., Adam, A. A., Wihastuti, T. A., & Rohman, M. S. (2017). Elaborate evaluation of serum and tissue oxidized LDL level with darapladib therapy: A feasible diagnostic marker for early atherogenesis. Asian Pacific Journal of Tropical Biomedicine, 7(2), 134-138. Assay: HDL and LDL/VLDL in sprague dewley rats serum.

Sadowska, J., Gebczynski, A. K., & Konarzewski, M. (2017). Metabolic risk factors in mice divergently selected for BMR fed high fat and high carb diets. PloS one, 12(2), e0172892. Assay: HDL and LDL/VLDL in mice serum.

Kho, M., Lee, Y., Park, J., Kim, H., Yoon, J., Ahn, Y. & Kang, D. (2016). Fermented red ginseng potentiates improvement of metabolic dysfunction in metabolic syndrome rat models. Nutrients, 8(6), 369. Assay: HDL and LDL/VLDL in sprague dewley rats plasma.

Bourdon JA et al (2012) Hepatic and pulmonary toxicogenomic profiles in mice intratracheally instilled with carbon black nanoparticles reveal pulmonary inflammation, acute phase response, and alterations in lipid homeostasis. Toxicol Sci 127(2):474-484. Assay: HDLandLDL/VLDL in mouse plasma.

Drew, B.G. et al (2011). Reconstituted High-density Lipoprotein Infusion Modulates Fatty Acid Metabolism in Patients with Type 2 Diabetes Mellitus. J Lipid Res. 52(3):572-81. Assay: HDL and LDL/VLDL in human blood.

Klusonova P, et al (2011). Local metabolism of glucocorticoids in Prague hereditary hypertriglyceridemic rats – Effect of hypertriglyceridemia and gender. Steroids 76(12):1252-9. Assay: HDL and LDL/VLDL in mouse blood.

Nagajyothi F et at (2011). Trypanosoma cruzi utilizes the host low density lipoprotein receptor in invasion. PLoS Negl Trop Dis. 1;5(2):e953. Assay: HDL and LDL/VLDL in mouse serum.

Parveen, T.D. et al (2011). Antihyperlipidemic Activity of the Methanolic extract from the Stems of Tinospora cordifolia on Sprague dawley rats. Der Pharmacia Sinica 2 (1): 104-109. Assay: HDL and LDL/VLDL in rat blood.

Seo, C-W. et al (2011). Antihyperlipidemic and Body Fat-Lowering Effects of Silk Proteins with Different Fibroin/Sericin Compositions in Mice Fed with High Fat Diet. J. Agric. Food Chem. 59 (8): 4192-4197. Assay: HDL and LDL/VLDL in mouse blood.

Takakuwa Y, et al (2011). AC13, a C-terminal fragment of apolipoprotein A-I, is a candidate biomarker for microscopic polyangiitis. Arthritis Rheum. 63(11):3613-24. Assay: HDL and LDL/VLDL in human blood.

Uddin, M.J. et al (2011). Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genetics 2011, 12:62. Assay: HDL and LDL/VLDL in pig serum.

Wan, W. et al (2011). Genetic Deletion of Chemokine Receptor Ccr6 Decreases Atherogenesis in ApoE-Deficient Mice. Circ Res.109(4):374-81. Assay: HDL and LDL/VLDL in mouse serum.

Oliver SR, et al (2010). Increased oxidative stress and altered substrate metabolism in obese children. Int J Pediatr Obes. 2010 Oct;5(5):436-44. Assay: HDL and LDL/VLDL in human plasma.

Ponda, M.P. et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210 (1): 57-62. Assay: HDL and LDL/VLDL in mouse blood.

Shanmugasundaram R, Selvaraj RK (2010). Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr (Berl). [Epub ahead of print]. Assay: HDLandLDL/VLDL in bird quail blood.

Tam J, et al (2010). Peripheral CB1 cannabinoid receptor blockade improves cardiometabolic risk in mouse models of obesity. J Clin Invest. 120(8):2953-66. Assay: HDL and LDL/VLDL in mouse serum.

Tucci, S. et al (2010). Medium-chain triglycerides impair lipid metabolism and induce hepatic steatosis in very long-chain acyl-CoA dehydrogenase (VLCAD)-deficient mice. Mol. Gen. Met. 101(1): 40-47. Assay: HDL and LDL/VLDL in mouse blood.

Zhu, F.G. et al (2010). Use of Inhibitors of Toll-like receptors in the prevention and treatment of hyperchloesterolenia and hyperlipidemia and dreases related thereto. US2010/0098685A1. Assay: HDL and LDL/VLDL in mouse blood.

Lee, S.M. et al (2008).GCG-rich tea catechins are effective in lowering cholesterol and triglyceride concentrations in hyperlipidemic rats. Lipids 43(5): 419-429. Assay: HDL and LDL/VLDL in rat blood.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.

AF HDL and LDL/VLDL Assay Kit
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For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.

Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt

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