EnzyChrom™ Glycerol Assay Kit

Protocol SDS

Application

• For quantitative determination of glycerol and evaluation of drug effects on glycerol metabolism.

Key Features

• Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 10 to 1000 µM (92 µg/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 µM for fluorimetric assays.
• Simple and convenient. The procedure involves the addition of a single working reagent and incubation for 20 min at room temperature, compatible with HTS assays.
• Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.

Method

• OD570nm, or FL530/585nm

Samples

• Biological, food, beverage, etc

Species

• All

Procedure

• 20 min

Size

• 200 tests

Detection Limit

• 2 µM

Shelf Life

• 12 months

More Details

Glycerol [Glycerin or Glycerine, C₃H₅ (OH) ₃] is widely used in foods, beverages, and pharmaceutical formulations. It is also a main by-product of biodiesel production. Simple, direct, and automation-ready procedures for measuring glycerol concentrations find wide applications. BioAssay Systems glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase, and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to glycerol concentration in the sample.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Li, C et al (2020). Matrix Gla protein regulates adipogenesis and is serum marker of visceral adiposity. Adipocyte, 9(1), 68-76. Assay: Glycerol in mouse cells.

Dowling, M et al (2020). Characterization of high fludioxonil resistance in botrytis cinerea isolates from calibrachoa flowers. Phytopathology, PHYTO-07-20-0268-R. Assay: Glycerol in botrytis cinerea.

Fontvieille, A et al (2019). Acute effect of post-resistance exercise milk-based supplement on substrate oxidation and fat mobilization in older men: A pilot study. Advances in Geriatric Medicine and Research, 1(2). Assay: Glycerol in human plasma.

Li, X et al (2020). Critical role of matrix metalloproteinase 14 in adipose tissue remodeling during obesity. Molecular and Cellular Biology, 40(8). Assay: Glycerol in mouse plasma.

Castillo, F., Gonzalez, D. R., & Moore-Carrasco, R. (2019). Effects of Phaseolus vulgaris Extract on Lipolytic Activity and Differentiation of 3T3-L1 Preadipocytes into Mature Adipocytes: A Strategy to Prevent Obesity. Journal of Nutrition and Metabolism, 2019. Assay: Glycerol in mouse cells.

Pan, Y., Zhao, W., Zhao, D., Wang, C., Yu, N., An, T. & Gu, Y. (2018). Salvianolic acid B improves mitochondrial function in 3T3-L1 adipocytes through a pathway involving PPARgamma coactivator-1alpha (PGC-1alpha). Frontiers in pharmacology, 9:671. Assay: Glycerol in mouse cells.

Sharma, V., Smolin, J., Nayak, J., Ayala, J. E., Scott, D. A., Peterson, S. N., & Freeze, H. H. (2018). Mannose Alters Gut Microbiome, Prevents Diet-Induced Obesity, and Improves Host Metabolism. Cell reports, 24(12), 3087-3098. Assay: Glycerol in mice serum.

Zhao, Y., Li, X., Yang, L., Eckel-Mahan, K., Tong, Q., Gu, X. & Sun, K. (2018). Transient overexpression of VEGF-A in adipose tissue promotes energy expenditure via activation of the sympathetic nervous system. Molecular and cellular biology, MCB-00242. Assay: Glycerol in mice tissues.

Loyola-Machado, A. C., Azevedo-Martins, A. C., Catta-Preta, C. M. C., de Souza, W., Galina, A., & Motta, M. C. M. (2017). The symbiotic bacterium fuels the energy metabolism of the host trypanosomatid Strigomonas culicis. Protist, 168(2), 253-269. Assay: Glycerol in bacteria (S. culicis) cells.

Allsopp, P., Crowe, W., Bahar, B., Harnedy, P. A., Brown, E. S., Taylor, S. S. & Strain, C. R. (2016). The effect of consuming Palmaria palmata-enriched bread on inflammatory markers, antioxidant status, lipid profile and thyroid function in a randomised placebo-controlled intervention trial in healthy adults. European journal of nutrition, 55(5), 1951-1962. Assay: Glycerol in mouse cells.

Burch, T. A. (2016). The Compatible Solute Glycerol as a Photosynthetic Sink and Energy Carrier in Freshwater and Marine Chlamydomonas. Assay: Glycerol in Algae cells.

Coughlin, David J., et al (2016). Elevated osmolytes in rainbow smelt: the effects of urea, glycerol and trimethylamine oxide on muscle contractile properties. Journal of Experimental Biology 219.7: 1014-1021. Assay: Glycerol in fish tissues.

Eastwood, L., Leterme, P., & Beaulieu, A. D. (2016). Body fat mobilization during lactation in high-producing sows fed varied omega-6 to omega-3 fatty acid ratios. Canadian Journal of Animal Science, 96(1), 69-78. Assay: Glycerol in sow serum.

Gallagher, H. L., Boule, M. A., & Zonfrelli, T. A. (2016). An Analytical Comparison of Ethanolysis and Methanolysis Kinetics and the Refined Biodiesel Product. Assay: Glycerol in alcohol and canola oil mixture.

Vadassery, J., Oelmuller, R., Dua, M., Johri, A. K., Nevo, E., Jogawat, A., & Verma, N. (2016). PiHOG1, a stress regulator MAP kinase from the root endophyte fungus Piriformospora indica, confers salinity stress tolerance in rice plants. Sci Rep. 6:36765. Assay: Glycerol in yeast cells.

Zhao, Y., Gu, X., Zhang, N., Kolonin, M. G., An, Z., & Sun, K. (2016). Divergent functions of endotrophin on different cell populations in adipose tissue. American Journal of Physiology-Endocrinology and Metabolism, 311(6), E952-E963. Assay: Glycerol in mouse cells.

Calamita G et al (2012). Biophysical assessment of aquaporin-9 as principal facilitative pathway in mouse liver import of glucogenetic glycerol. Biol Cell 104(6):342-51. Assay: Glycerol in mouse plasma.

Bahar, B., et al. (2011). A potential role of IL-6 in the chito-oligosaccharide-mediated inhibition of adipogenesis. Br J Nutr. 106(8): 1142-53. Assay: Glycerol in mouse 3T3-L1 cells.

Drew, B.G., et al. (2011). Reconstituted high-density lipoprotein infusion modulates fatty acid metabolism in patients with type 2 diabetes mellitus. J Lipid Res 52(3):572-81. Assay: Glycerol in human plasma.

Pifferi, F., et al. (2011). Mild experimental ketosis increases brain uptake of 11C-acetoacetate and 18F-fluorodeoxyglucose: a dual-tracer PET imaging study in rats. Nutr Neurosci 14(2):51-8. Assay: Glycerol in rat plasma.

Wachtler, B., et al. (2011). From attachment to damage: defined genes of Candida albicans mediate adhesion, invasion and damage during interaction with oral epithelial cells. PLoS One 6(2):e17046. Assay: Glycerol in Candida albicans fungus.

Lung, M., et al. (2009). Diacylglycerol kinase epsilon is selective for both acyl chains of phosphatidic acid or diacylglycerol. J Biol Chem 284(45):31062-73. Assay: Glycerol in diacylglycerol.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.