QuantiChrom™ FRAP Assay Kit

Protocol SDS

Application

• For quantitative determination of ferric reduction antioxidant potential (FRAP).

Key Features

• Sensitive and accurate. Linear detection range: 0.5 to 180 µM Fe³⁺ reduction potential in a 96-well plate assay.
• Simple and high-throughput. The procedure involves the addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
• Improved reagent stability and versatility. The optimized formulation has greatly enhanced the reagent and signal stability.

Method

• OD590nm

Samples

• Plant extracts, foods, vitamins, supplements, and biological samples such as serum, plasma, and urine

Species

• All

Procedure

• 40 min

Size

• 250 tests

Detection Limit

• 0.5 µM

Shelf Life

• 12 months

More Details

FERRIC REDUCTION ANTIOXIDANT POTENTIAL (FRAP) is a measure of antioxidant capacity quantified by the antioxidant potential to reduce ferric iron (III) to ferrous iron (II). Antioxidants protect cells from damage by reactive oxygen species that are produced by oxidation reactions. As oxidative stress contributes to the development of many diseases including Alzheimer’s disease, Parkinson’s disease, diabetes, rheumatoid arthritis, and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Antioxidants are also widely used as dietary supplements and as preservatives in a wide range of products such as food, cosmetics, rubber, and gasoline.Simple, direct and high-throughput assays for antioxidant capacity find wide applications in research, the food industry, and drug discovery. BioAssay Systems’ improved assay measures antioxidant potential in which Fe³⁺ is reduced by antioxidant to Fe²⁺. The resulting Fe²⁺ specifically forms a colored complex with a chromogen. The color intensity at 590 nm is proportional to FRAP in the sample.

Can you use this assay with serum or plasma samples?

Yes, serum and plasma samples should be diluted 10-fold in dH₂O prior assay. Correct for dilution factor (n = 10) in the calculation.

Can you use this assay with urine samples?

Yes, urine samples should be diluted 20-fold in dH₂O prior assay. Correct for dilution factor (n = 20) in the calculation.

What happens if my sample has oxidants or reactive oxygen species (ROS) in it?

Samples with oxidants or ROS will yield lower measurements of antioxidant potential because antioxidants in the sample will reduce the oxidants and ROS rather than the Fe³⁺, this results in lower levels of Fe²⁺,which is what the assay measures to determine FRAP.

What happens if my sample has iron in it?

This assay measures antioxidant potential to reduce Fe³⁺ to Fe²⁺. If the sample has intrinsic Fe²⁺, this will yield a false positive for antioxidant potential.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.