QuantiFluo™ DNA Assay Kit

Application

• For quantitative determination of DNA.

Key Features

• Sensitive and accurate. Linear detection range 2 ng to 40 ng (100 – 2,000 ng/mL) calf thymus DNA in 96-well plate assay.
• Simple and high-throughput. The “mix-and-read” procedure involves addition of a single working reagent and reading the fluorescence intensity. Can be readily automated as a high-throughput assay for thousands of samples per day.
• Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay.

Method

• FL350/450nm

Samples

• Plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices

Species

• All

Procedure

• 5 min

Size

• 250 tests

Detection Limit

• 100 ng/mL

Shelf Life

• 12 months

More Details

Can you guarrantee, that your kit enables an accurate estimation of cDNA concentration in RT reaction mixture, where high excess of total RNA is present? Is it a SYBR Green based method?

We are using Hoechst dye based DNA assay. It is very specific for DNA and not RNA. However, as we do not know the RNA content in your sample, we can not judge if it would meet your needs.

Mayer-Wagner S, et al (2011). Effects of low frequency electromagnetic fields on the chondrogenic differentiation of human mesenchymal stem cells. Bioelectromagnetics. 32(4):283-90. Assay: DNA in human stem cell.

Waheed MM et al (2011). Some Biochemical Characteristics and Preservation of Epididymal Camel Spermatozoa (Camelus dromedarius). Theriogenology 2011 Jul 13. Assay: DNA in human cell line.

Labuzek K, et al (2010). Metformin has adenosine-monophosphate activated protein kinase (AMPK)-independent effects on LPS-stimulated rat primary microglial cultures. Pharmacol Rep. 62(5):827-48. Assay: DNA in rat microglial cells.

Labuzek K, et al (2010). Ambivalent effects of compound C (dorsomorphin) on inflammatory response in LPS-stimulated rat primary microglial cultures. Naunyn Schmiedebergs Arch Pharmacol. 381(1):41-57. Assay: DNA in rat primary glial culture.

Mayer-Wagner S et al. (2010). Membrane-based cultures generate scaffold-free neocartilage in vitro: influence of growth factors. Tissue Eng Part A. 16(2):513-21. Assay: DNA in human cell.

Bhavnani B.R. et al. (2008). Structure activity relationships and differential interactions and functional activity of various equine estrogens mediated via estrogen receptors (ERs) ERalpha and ERbeta. Endocrinology. 149(10):4857-70. Assay: DNA in human cell line.

Lai, M et al (2007). Development of an In-Vitro Screening Method to Determine Enzymatic Release rates from a Homologous Series of Novel SN-38 Conjugates. Sonus Pharmaceuticals. 2007 AAPS Annual Meeting & Exposition. Assay: DNA in human cells, stem cells.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.