This kit is shipped at room temperature. Upon receiving, please keep the kit in the freezer (-20 to -10°C) for long term stability.
Serum samples were tested. Since DAO is copper dependent, EDTA should be avoided in sample preparation.
Serum and plasma samples greatly influence the HRP reaction. In order to measure an appropriate RFU for H2O2 in a serum or plasma matrix, an internal standard is required.
Yes, the assay can be used in any standard black plate or cuvette. Simply adjust the total reaction volume to your container. For 384 well use 20-90 μL, for a cuvette use 1 mL (some narrow cuvettes can hold 200-500 μL).
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Working Reagents should be made fresh for each assay and used within 1 hour.
Typically if you are within ± 20 nanometers the assay will still work, but have slightly reduced sensitivity. Some assays have a narrower peak than others, call our tech support for a spectrum to verify.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt
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