EnzyChrom™ D-Lactate Assay Kit

Protocol SDS

Application

• For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

Key Features

• Sensitive and accurate. The detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in a 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

• OD565 nm

Samples

• Serum, plasma, cell culture media, etc

Species

• All

Procedure

• 20 min

Size

• 100 tests

Detection Limit

• 0.05 mM

Shelf Life

• 6 months

More Details

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct, and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

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Lee, JH et al (2020). Effect of cysteine on methylglyoxal-induced renal damage in mesangial cells. Cells, 9(1). Assay: D-Lactate in mouse and human cells.

Aranda-Diaz, A et al (2020). Bacterial interspecies interactions modulate pH-mediated antibiotic tolerance. ELife, 9. Assay: D-Lactate in lactobacillus plantarum with acetobacter culture supernatant.

Lee, J. H. et al (2020). Effect of Cysteine on Methylglyoxal-Induced Renal Damage in Mesangial Cells. Cells, 9(1). Assay: D-lactate in human MES13 cells.

Aranda-Diaz, A. et al (2020). Bacterial Interspecies Interactions Modulate pH-mediated Antibiotic Tolerance. eLife, 9, e51493. Assay: D-lactate in acetobacter cell lysate.

Kang, K. et al (2020). Mesenchymal Stromal Cells From Patients With Cyanotic Congenital Heart Disease Are Optimal Candidate for Cardiac Tissue Engineering. Biomaterials, 230, 119574. Assay: D-lactate in human hMSC culture medium.

Aranda-Diaz, Andres, et al (2019). Bacterial interspecies interactions modulate pH-mediated antibiotic tolerance in a model gut microbiota. bioRxiv: 538132. Assay: D-Lactate in bacteria culture.

Kwan, Billy KY, et al (2017). Marine microalgae as dietary supplements in the culture of juvenile Chinese horseshoe crabs, Tachypleus tridentatus (Xiphosura). Aquaculture research 48.7: 3910-3924. Assay: D-Lactate in horseshoe crabs plasma.

Marcu, R et al (2014). Mitochondrial Matrix Ca2 Accumulation Regulates Cytosolic NAD/NADH Metabolism, Protein Acetylation, and Sirtuin Expression. Mol Cell Biol. 34(15):2890-902. Assay: D-Lactate in human endothelial cells.

Raykov, Z et al (2014). Myo-inositol trispy rophosphate-mediated hypoxia reversioncontr ols pancreatic cancer in rodents and enhances gemcitabineefficacy. Int J Cancer. 134(11):2572-82. Assay: D-Lactate in pancreatic tumor cells in rats and mice.

Raykov, Z et al (2014). Myo-inositol trispyrophosphate-mediated hypoxia reversion controls pancreatic cancer in rodents and enhances gemcitabineefficacy. Int J Cancer. 134(11):2572-82. Assay: D-Lactate in rats and mice pancreatic tumor cells.

Guzman, FJ, et al (2013). Temporal relationship of serum markers and tissue damage during acute intestinal ischemia/reperfusion. Clinics (Sao Paulo). 68(7):1034-8. Assay: D-Lactate in rat serum.

Witkin SS., et al (2013). Influence of vaginal bacteria and D- and L-lactic acid isomers on vaginal extracellular matrix metalloproteinase inducer: implications for protection against upper genital tract infections. MBio. 6;4(4). pii: e00460-13. Assay: D-Lactate in human vaginal secretions.

Gigante A et al (2012) D-Lactic acidosis 25 years after bariatric surgery due to Salmonella enteritidis. Nutrition 28(1):108-11. Assay: D-Lactate in human serum.

Kurundkar, A.R., et al. (2010). Extracorporeal membrane oxygenation causes loss of intestinal epithelial barrier in the newborn piglet. Pediatr Res 68(2):128-33. Assay: D-Lactate in pig plasma.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.