EnzyLight™ Cytotoxicity Assay Kit

Protocol SDS

Application

• For rapid, bioluminescent luciferin/luciferase-based assay for cell viability, proliferation, cytotoxicity, and high-throughput screen for anticancer agents.

Key Features

• Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).
• Sensitive and accurate. As low as 50 cells can be quantified.
• Homogeneous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
• Robust and amenable to HTS: Z factors of 0.6 to 0.7 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• Luminescence

Samples

• Cells

Species

• All

Procedure

• 2 min

Size

• 100 tests

Detection Limit

• 50 cells

Shelf Life

• 6 months

More Details

Adenosine 5’-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency” of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery. BioAssay Systems’ EnzyLight™ Cytotoxicity Assay Kit provides a rapid method to measure intracellular ATP, cell viability and cytotoxicity. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration and hence number of living cells. This non-radioactive, homogeneous cell-based assay can be conveniently performed in microplates. The reagent is compatible with all culture media and liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Massa, S.M., et al. (2010). Small molecule BDNF mimetics activate TrkB signaling and prevent neuronal degeneration in rodents. J Clin Invest 120(5):1774-85. Assay: Cytotoxicity in mouse fibroblast cells.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.