This is completely normal. The assay is measuring the decrease in absorbance at 500nm. The higher the concentration of biotin, the lower the absorbance will be in the calculation.
The biotin levels in your sample may be significantly greater than the kit detection limit. We recommend diluting your sample and assaying again. You may wish to perform a serial dilution on the sample to determine the optimal dilution factor. Aim for a dilution that yields a concentration read out somewhere in the middle of the standard curve. Correct for the dilution factor in the calculation.
This is likely indicative no biotin or biotin below the kit detection limit.
Yes, the kit is shipped at room temperature and is stable for the duration of shipment at that temperature. Upon receipt, the kit should be stored at -20°C.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
For orders of 10 or more kits, please call +1-510-782-9988×1 or email us for best pricing and/or bulk order.
Orders are shipped the same day if placed by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt
Technical support provided by the very scientists that develop the assays.
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.
Because we develop and manufacture the products, our prices are lower than competitors on the market
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