EnzyChrom™ Ascorbic Acid Assay Kit

Protocol SDS

Application

• For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism.

Key Features

• Use 20 µL samples. Linear detection range: colorimetric assay 6 to 1000 µM, fluorimetric assay 1 to 100 µM ascorbic acid.

Method

• OD570nm, or FL530/585nm

Samples

• Serum, plasma, urine, saliva, milk, tissue, and cell culture

Species

• All

Procedure

• 10 min

Size

• 100 tests

Detection Limit

• OD, FL: 6, 1 µM

Shelf Life

• 6 months

More Details

Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. BioAssay Systems method provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H₂O₂ which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.

We would like to measure ascorbic acid in liver homogenates, which are in 0.25M Sucrose. We want to confirm the sucrose will interfere with the assay?

We have not tested the compatibility of sucrose with this assay. Based on the mechanism of the assay we see no reason why sucrose would interfere and believe the assay will work just fine.

Susanto, L., Y. Siregar, and L. Kusumawati (2018). Vitamin C supplementation improve the sputum conversion culture rate in pulmonary tuberculosis treatment while rifampicin susceptible. IOP Conference Series: Earth and Environmental Science 125(1). Assay: Ascorbic acid in human plasma.

Kantar, Michael B., et al (2016). Vitamin variation in Capsicum Spp. provides opportunities to improve nutritional value of human diets. PloS one 11.8: e0161464. Assay: Ascorbic acid in pepper tissue.

Trezzi, Jean-Pierre, et al (2016). LacaScore: a novel plasma sample quality control tool based on ascorbic acid and lactic acid levels. Metabolomics 12.6: 96. Assay: Ascorbic acid in human plasma.

Sasaki, M. et al (2012). D-2-hydroxyglutarate produced by mutant IDH1 perturbs collagen maturation and basement membrane function. Genes & Dev. 26: 2038-2049. Assay: Ascorbic acid in mouse cell.

Ching-Shih Chen et al (2011). Thiazolidinedione Energy Restriction-Mimetic Agents. US 2011/0086895 Al. Assay: Ascorbic acid in mouse cell.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.