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QuantiFluo™ Angiotensin-Converting Enzyme 1 Assay Kit
Application
- For quantitative determination of ACE-1 activity determination in biological samples.
Key Features
- Safe and convenient. Non-radioactive assay. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
- Sensitive and accurate. Linear detection range 0.01 – 5 U/L ACE-1 in a 96-well plate assay.
- High-throughput. Can be readily automated to assay thousands of samples per day.
Method
- FL360/485nm
Samples
- Serum, cell or tissue lysate, and other biological samples
Species
- All
Procedures
- 45 min
Size
- 100 tests in 96-well plate
Detection Limit
- 0.01 U/L
Shelf Life
- 6 months
More Details
ANGIOTENSIN-CONVERTING ENZYME 1, ACE-1 (EC 3.4.15.1) is a critical enzyme in the renin-angiotensin-aldosterone system (RAAS), which regulates blood pressure, fluid, and electrolyte balance in the body. Found primarily in the lung endothelium and kidney epithelial cells, ACE-1 hydrolyzes angiotensin I, cleaving off the His-Leu dipeptide to produce angiotensin II. Its role in the RAAS system makes it a major target for hypertension and cardiovascular treatments.BioAssay Systems’ EACE1-100 Kit provides a convenient fluorometric method to measure ACE-1 activity in biological samples. In this assay, ACE-1 hydrolyzes the synthetic substrate, releasing the product. Then, o-phthalaldehyde (OPA) fluorescently labels the product for quantification. The increase in fluorescence at λex/em = 360/485 nm is directly proportional to enzyme activity.My Standard component appears to contain precipitate upon thawing to room temperature. Has this been observed before?
Yes, this is actually quite common. Vortex the Standard and the solid should quickly dissolve. The Standard has undergone many freeze/thaw cycles with this same observation and results are always consistent.
Would it be possible to combine the Working Reagent and Detection Reagent into one reagent and read for 45 minutes instead of separating the steps?
No, the mechanism behind this reaction includes two parts. The first step involves the addition of Working Reagent with Substrate with the enzyme catalyzing the reaction. The second step uses the Detection Reagent to stop the reaction and detect the product. Both steps are necessary and must be carried out separately.
Does this kit measure angiotensin-converting enzyme 2 (ACE2) in addition to angiotensin-converting enzyme 1 (ACE1)?
This kit is specific to ACE1 due to the differences in substrate specificity between ACE1 and ACE2.
For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
$419.00
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.
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