QuantiChrom™ α-Amylase Assay Kit
- For quantitative determination of α-amylase activity in biological samples
- Safe. Non-radioactive assay.
- Sensitive and accurate. Linear detection range of 0.2 – 100 U/L α-amylase in a 96-well plate assay.
- Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.
- Biological samples
- 20 min
- 100 tests in 96-well plate
- 0.2 U/L
- 6 months
More Detailsα-AMYLASE (EC 188.8.131.52) belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4-glycosidic bonds. The α-amylases cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, as well as glucose and “limit dextrin” from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased α-amylase levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis and renal failure. Simple, direct and automation-ready procedures for measuring amylase activity are very desirable. BioAssay Systems’ QuantiChrom™ α-Amylase Assay Kit provides a convenient colorimetric method to measure α-amylase activity in biological samples. In this assay, α-amylase hydrolyzes a synthetic substrate to release 2-chloro-4-nitrophenol. The rate of formation of the 2-chloro-4-nitrophenol, measured at 405 nm, is directly proportional to the enzyme activity.
Does this kit come with α-amylase?
This kit does not come with α-amylase. Instead, the customer will need to purchase their own enzyme if desired. The following protocol is optimized for porcine α-amylase from Calzyme (Cat #146A0100).
How is the standard curve used to quantify α-amylase in the sample?
The mechanism of this kit is based on the reaction of α-amylase with a synthetic substrate to yield a 2-chloro-4-nitrophenol product that is measurable at OD405nm. The nitrophenol standard is measured similarly to the product of the reaction and is therefore proportional to the concentration of product released.
I know that α-amylase and α-glucosidase are both necessary to digest starch and other carbohydrates into glucose. Does this kit require the use of α-glucosidase when reacting with the synthetic substrate?
The synthetic substrate is specific to α-amylase and should not require the use of α-glucosidase nor see interference from it.
For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.
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