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QuantiFluo™ Ammonia Assay Kit II


  • For quantitative determination of ammonia and ammonium in biological samples.

Key Features

  • Fast and sensitive. Linear detection range of 0.005 – 2mM ammonia.
  • Convenient and high-throughput. A single component reagent. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.


  • FL415/475 nm


  • Serum, Plasma, urine, and other biological samples


  • All


  • 20 min


  • 100 tests

Detection Limit

  • 5.3 µM

Shelf Life

  • 6 months

More Details

AMMONIA (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH3 are very desirable. BioAssay Systems’ updated ammonia/ammonium assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 415/475nm) is proportional to the ammonia concentration in the sample.

For more detailed product information and questions, please feel free to email us or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.

Ammonia Assay Kit II
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For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.

Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: FedEx
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.

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