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Acetylcholinesterase Reagent
Application
- Direct assays of acetylcholinesterase activity in blood, serum, plasma, and other biological samples.
Key Features
- Simple and Convenient. This single reagent is ready to use, when reconstituted in 25mL deionized water.
- High-throughput. Can be readily adapted on chemistry analyzers or high-throughput liquid handling system to test thousands of samples per day.
Method
- Ellman method, OD410nm
Samples
- whole blood, serum, plasma etc
Species
- All
Procedures
- 10 min
Size
- 100 tests
Detection Limit
- 6 U/L
Shelf Life
- 6 months
More Details
ACETYLCHOLINESTERASE (EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. Low serum cholinesterase activity may relate to exposure to insecticides or to one of a number of variant genotypes. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Cholinesterase levels of cells and plasma are used as a guide in establishing safety precautions relative to exposure and contact, as well as a guide in determining the need for workers to be removed from areas of contact with the organic phosphate insecticides.Simple, direct and automation-ready procedures for measuring AChE activity are very desirable. BioAssay Systems’ acetylcholinesterase reagent is based on an improved Ellman method, in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5′-dithiobis(2-nitrobenzoic acid). The intensity of the product color at 410 nm is proportionate to the enzyme activity in the sample.
What is the difference between this reagent and your DACE-100 assay kit?
This is a single reagent that has been optimized for use on both Chemistry Analyzer and in 96-well plate assay systems. The reagent is convenient and before use, only needs to mix with 25mL distilled or deionized water.
Does the assay detect only acetylcholinesterase activty?
No, this assay detects acetylcholinesterase and butyrylcholinesterase (also known as plasma cholinesterase, pseudocholinesterase) and does not distinguish between the two.
Which anticoagulant should I use for blood sample collection?
We recommend using serum for measuring plasma cholinesterase activity and EDTA blood for measuring red blood cell acetylcholinesterase activity.
For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
$89.00
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: 2-25°C upon receipt.
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