Protocol SDS

Application

• Direct assays of acetylcholinesterase activity in blood, serum, plasma, and other biological samples.

Key Features

• Simple and Convenient. This single reagent is ready to use, when reconstituted in 25mL deionized water.
• High-throughput. Can be readily adapted on chemistry analyzers or high-throughput liquid handling system to test thousands of samples per day.

Method

• Ellman method, OD410nm

Samples

• whole blood, serum, plasma etc

Species

• All

Procedures

• 10 min

Size

• 100 tests

Detection Limit

• 6 U/L

Shelf Life

• 6 months

More Details

ACETYLCHOLINESTERASE (EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. Low serum cholinesterase activity may relate to exposure to insecticides or to one of a number of variant genotypes. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Cholinesterase levels of cells and plasma are used as a guide in establishing safety precautions relative to exposure and contact, as well as a guide in determining the need for workers to be removed from areas of contact with the organic phosphate insecticides.

Simple, direct and automation-ready procedures for measuring AChE activity are very desirable. BioAssay Systems’ acetylcholinesterase reagent is based on an improved Ellman method, in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5′-dithiobis(2-nitrobenzoic acid). The intensity of the product color at 410 nm is proportionate to the enzyme activity in the sample.