EnzyChrom™ Ketone Body Assay Kit

Application

• For quantitative determination of ketone body (acetoacetate and 3-hydroxybutyrate) and evaluation of drug effects on its metabolism.

Key Features

• Sensitive and accurate. Uses a 10 µL sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent and reading the optical density at room temperature.
• High-throughput. Can be automated as a high-throughput 96-well plate assay for many samples per day.

Method

• OD340nm

Samples

• Serum, plasma, urine etc

Species

• All

Procedure

• 30 min

Size

• 200 tests

Detection Limit

• 0.12 mM

Shelf Life

• 6 months

More Details

Can serum and plasma samples be stored frozen?

β-hydroxybutyrate is stable and can be detected in samples that had been stored frozen for more than a month. Acetoacetate (AcAc), on the other hand, is an unstable compound which becomes rapidly decarboxylated. We recommend to use fresh serum or freeze serum samples at -80°C for up to one month.

I seem to be getting very low levels of BOH and AcAc in freshly drawn blood. Perhaps this is because they are breaking down within the hour it takes me to process the plasma out.

In healthy individuals, normal blood values for ketone bodies are actually very low or not detectable. Elevated blood levels of ketone bodies (ketosis) can be caused for example by diabetes, alcohol intoxication, starvation, or physical exercise. You can check for the stability of your analyte by setting aside an aliquot of your sample and add the standard as a positive control.

Moreno, D et al. (2020). Vulnerability of the endangered Maugean Skate population to degraded environmental conditions in Macquarie Harbour. Assay: Ketone body in maugean skate plasma.

Lin, X et al. (2020). Effects of dietary anaplerotic and ketogenic energy sources on renal fatty acid oxidation induced by clofibrate in suckling neonatal pigs. International Journal of Molecular Sciences, 21(3), 726. Assay: Ketone body in porcine kidney.

Wallenius, V et al. (2020). Suppression of enteroendocrine cell glucagon-like peptide (Glp)-1 release by fat-induced small intestinal ketogenesis: A mechanism targeted by Roux-en-Y gastric bypass surgery but not by preoperative very-low-calorie diet. Gut, 69(8), 1423-1431. Assay: Ketone body in human serum.

Khalil, HMA et al. (2020). Edible dairy formula fortified with coconut oil for neuroprotection against aluminium chloride-induced Alzheimers disease in rats. Journal of Functional Foods, 104296. Assay: Ketone body in rat serum.

Umbarawan, Y et al. (2020). Reduced fatty acid uptake aggravates cardiac contractile dysfunction in streptozotocin-induced diabetic cardiomyopathy. Scientific Reports, 10(1), 20809. Assay: Ketone body in mouse serum.

Moorhead, SG et al. (2020). Variation of body condition and plasma energy substrates with life stage, sex, and season in wild-sampled nurse sharks Ginglymostoma cirratum. Journal of Fish Biology. Assay: Ketone body in shark plasma.

Cui, A., Fan, H., Zhang, Y., Zhang, Y., Niu, D., Liu, S. & Liu, Y. (2019). Dexamethasone-induced Kruppel-like factor 9 expression promotes hepatic gluconeogenesis and hyperglycemia. The Journal of clinical investigation 130:2266-2278. Assay: Ketone body in mice serum.

Saito, T., Kuma, A., Sugiura, Y., Ichimura, Y., Obata, M., Kitamura, H. & Saito, I. (2019). Autophagy regulates lipid metabolism through selective turnover of NCoR1. Nature communications 10(1):1567. Assay: Ketone body in mice plasma.

Herrick, K. J., Hippen, A. R., Kalscheur, K. F., Schingoethe, D. J., Ranathunga, S. D., Anderson, J. L. & Van Eys, J. E. (2018). Infusion of butyrate affects plasma glucose, butyrate, and beta-hydroxybutyrate but not plasma insulin in lactating dairy cows. Journal of dairy science, 101(4), 3524-3536. Assay: Ketone body in cow plasma.

Iso, T., Haruyama, H., Sunaga, H., Matsui, H., Matsui, M., Tanaka, R. & Hanaoka, H. (2018). CD36 is indispensable for nutrient homeostasis and endurance exercise capacity during prolonged fasting. Physiological reports, 6(19), e13884. Assay: Ketone body in mice serum.

Lyons, K. (2018). Physiological consequences of environmental contamination in an elasmobranch with matrotrophic histotrophy, the Round Stingray (Urobatis halleri). Assay: Ketone body in stingrays plasma.

Bisgaier, Charles L., and Daniela Carmen Oniciu (2017). Treatment of NASH with gemcabene. U.S. Patent No. 9,849,104 Assay: Ketone body in mice plasma.

Goossens, C., Marques, M. B., Derde, S., Vander Perre, S., Dufour, T., Thiessen, S. E. & De Bock, K. (2017). Premorbid obesity, but not nutrition, prevents critical illness-induced muscle wasting and weakness. Journal of cachexia, sarcopenia and muscle, 8(1), 89-101. Assay: Ketone body in mice serum.

Herrick, K. J., Hippen, A. R., Kalscheur, K. F., Schingoethe, D. J., Casper, D. P., Moreland, S. C., & Van Eys, J. E. (2017). Single-dose infusion of sodium butyrate, but not lactose, increases plasma beta-hydroxybutyrate and insulin in lactating dairy cows. Journal of dairy science, 100(1), 757-768. Assay: Ketone body in cow plasma.

Driver, C. (2016). Metformin does not prevent glucose intolerance but improves renal function and reduces oxidative stress in type 1 diabetes (Doctoral dissertation). Assay: Ketone body in rat serum and urine.

Murunga, A. N., Miruka, D. O., Driver, C., Nkomo, F. S., Cobongela, S. Z., & Owira, P. M. (2016). Grapefruit derived flavonoid naringin improves ketoacidosis and lipid peroxidation in type 1 diabetes rat model. PloS one, 11(4), e0153241. Assay: Ketone body in rat serum and urine.

Neondo, M. A. (2016). The effects of naringin on glucose tolerance and ketoacidosis in type 1 diabetes (Doctoral dissertation). Assay: Ketone body in rat serum and urine.

Syamsunarno, MRAA et al (2014). Fatty Acid Binding Protein 4 and 5 Play a Crucial Role in Thermogenesis under the Conditions of Fasting and Cold Stress. PloS one 9.3: e90825. Assay: Ketone body in mouse serum.

Fujieda, Y et al (2013). Inflammation and Resolution Are Associated with Upregulation of Fatty Acid beta-Oxidation in Zymosan-Induced Peritonitis. PloS one 8.6: e66270. Assay: Ketone body in mouse liver tissue.

Martin-Murphy, BV et al (2013). Increased susceptibility of natural killer T-cell-deficient mice to acetaminophen-induced liver injury. Hepatology 57.4: 1575-1584. Assay: Ketone body in mouse serum.

Syamsunarno, MRAA et al (2013). A Critical Role of Fatty Acid Binding Protein 4 and 5 (FABP4/5) in the Systemic Response to Fasting. PloS one 8.11: e79386. Assay: Ketone body in mouse serum.

Qi S et al (2012). Comparison of the metabolic profiling of hepatitis B virus-infected cirrhosis and alcoholic cirrhosis patients by using (1) H NMR-based metabonomics. Hepatol Res 42(7):677-85. Assay: Ketone body in human serum.

Klusonova P et al (2011) Local metabolism of glucocorticoids in Prague hereditary hypertriglyceridemic rats–effect of hypertriglyceridemia and gender. Steroids 76(12):1252-9. Assay: Ketone Body in rat Hepatocyte culture.

Sharma NS et al (2011). Metabolic profiling based quantitative evaluation of hepatocellular metabolism in presence of adipocyte derived extracellular matrix. PLoS One 6(5):e20137. Assay: Ketone body in rat hepatocyte culture.

Sharma NS et al (2011). Metabolic profiling based quantitative evaluation of hepatocellular metabolism in presence of adipocyte derived extracellular matrix. PLoS One 6(5):e20137. Assay: Ketone Body in human serum.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.