Description
Application
- For evaluation of drugs and screening of potential inhibitors to MMP-1.
Key Features
- Robust. Fluorescence-based assay with a longer wavelength probe (λex/em = 490/520 nm), minimizing test compound interference. Robust assay with a Z’ factor of > 0.8.
- Homogeneous and convenient. Homogenous “mix-incubate-measure” assay. No wash and reagent transfer steps are involved.
- High-throughput. Can be readily automated to assay thousands of samples per day.
Method
- FL490/520 nm
Samples
- Compounds that affect MMP-1.
Species
- Human
Procedures
- 75 min
Size
- 100 tests (Custom bulk sizes available upon request)
Shelf Life
- 6 months
Screening Services
- BioAssay Systems offers comprehensive MMP-1 lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us by Service.
More Details
MATRIX METALLOPROTEINASE-1 or MMP-1 (EC 3.4.24.7) is a Ca2+– and Zn2+-dependent, interstitial collagenase that plays a role in the remodeling of the extracellular matrix of tissues. MMP-1 is up-regulated in metastatic cancer cells and in rheumatoid arthritis.
BioAssay Systems’ EnzyFluo™ MMP-1 Inhibitor Assay Kit uses a quenched synthetic substrate. Upon digestion by MMP-1, the substrate is cleaved, which abolishes the quenching of the fluorescence label. The fluorescent fragment is measured at λex/em = 490/520 nm. Inhibition is determined by the decrease in fluorescence.
