QuantiChrom™ Sialic Acid Assay Kit

Protocol SDS

Application

• For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on sialic acid.

Key Features

• Sensitive and accurate. Use as little as 60 µL samples. Linear detection range in 96-well plate: 5 to 1000 µM sialic acid for colorimetric assays and 0.5 to 100 µM for fluorimetric assays.

Method

• OD549nm, or FL555/585nm

Samples

• Serum, plasma, saliva, milk, etc

Species

• All

Procedure

• 2.5 hrs

Size

• 100 tests

Detection Limit

• OD, FL: 5, 0.5 µM

Shelf Life

• 24 months

More Details

SIALIC ACID is a general name for nine-carbon acidic sugars with N- or O-substituted derivatives. The most common member of these sugars is N-acetylneuraminic acid (NANA). Sialic acid is widely distributed throughout mammalian tissues and fluids including serum. Sialylated oligosaccharides have been shown to exhibit antiviral properties and are also known to influence blood coagulation and cholesterol levels. The sialic acid level in body fluids is also an important marker for diagnosing cancer. Simple and direct procedures for measuring sialic acid concentrations find wide applications in research and drug discovery. BioAssay Systems sialic acid assay uses an improved Warren method, in which sialic acid is oxidized to formylpyruvic acid which reacts with thiobarbituric acid to form a pink-colored product. The color intensity at 549 nm or fluorescence intensity at λex/em = 555/585 nm is directly proportional to the sialic acid concentration in the sample.

What is the difference between your two sialic acid assay kits?

The DSLA-100 assay kit is based on the oxidation of sialic acid with periodate and detection of the reaction product formyl pyruvic acid with thiobarbituric acid.The ESLA-100 assay kit is based on enzymatic digestion of sialic acid with NANA-aldolase (N-acetyl-neuraminic acid aldolase) and detection of the breakdown product pyruvate.

Esievo, K. A. N. et al (2021). Elevated serum sialic acids, a potent biomarker of alloxan-induced type 1 diabetes in dogs by ethanolic extract of Anogeissus leiocarpus. Journal of Diabetes & Metabolic Disorders, 1-8. Assay: sialic acid in dog serum.

Azeez, R. A (2018). The Related Changes Between Diabetic Positive Family History Children’S And Salivary Sialic Acid, Flow rate, Age, Gender In Iraqi Primary School. International Journal of Design and Nature 8(2):245-250. Assay: Sialic Acid in human saliva.

Taib, I.S. et al.(2015) “Palm oil tocotrienol-rich fraction attenuates testicular toxicity induced by fenitrothion via an oxidative stress mechanism.” Toxicology Research 4.1: 132-142. Assay: Sialic Acid in rat tissue.

Taib, IS et al (2015). Palm oil tocotrienol-rich fraction attenuates testicular toxicity induced by fenitrothion via an oxidative stress mechanism. Toxicology Research 4.1: 132-142. Assay: SialicAcid in rat tissue.

Gantner, M et all (2014). Partial synthesis of ganglioside and lysoganglioside lipoforms as internal standards for MS quantification. Journal of Lipid Research. 55, 2692-2704. Assay: Sialic Acid in bovine brain gangliosides.

Pawluczyk, Izabella ZA, et al (2014). “Sialic acid attenuates puromycin aminonucleoside-induced desialylation and oxidative stress in human podocytes.” Experimental cell research 320.2: 258-268. Assay: Sialic Acid in podocytes.

Chrostek, L et al (2013). Sialic acid levels reflects the disturbances of glycosylation and acute-phase reaction in rheumatic diseases. Rheumatology International. 34(3): 393-399. Assay: Sialic Acid in human serum.

Carvalho FA, et al (2011). Variations on fibrinogen-erythrocyte interactions during cell aging. PLoS One. 6(3):e18167. Assay: Sialic Acid in human blood.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.